LOCUS       sequence-96322-e        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse dysferlin transcript variant-2 (accession #NM_001077694) cDNA 
            in pDONR221 plasmid which can be used as an entry vector for 
            Invitrogen's gateway system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  TITLE     Mouse dysferlin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Mouse-Dysferlin-Transcript-Variant2
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1481..1492
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7039..7138)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7155..7174)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7156..7174)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7179..7195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7179..7195)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7297..7316)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7308..8117
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7375..7394)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8264..8852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8753..8772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca ctagtggtac cgtttaaacg atcggccgcc accatgctgc gagtcttcat
      721 cctttttgcg gagaatgtcc acaccccgga ctccgacatc agcgatgcct actgctccgc
      781 ggtgtttgca ggggtaaaga agagaaccaa agtcatcaag aacagtgtga accccgtgtg
      841 gaatgagggc tttgagtggg acctcaaagg tattcctcta gatcagagct cagaacttct
      901 cgtggtggtc aaggaccatg agacgatggg aagaaacagg ttcctggggg aagccaagat
      961 cccactccag gaggtccttg ccacccccag cctctctgcc agcttcaacg cacctctgct
     1021 agacgccaag cagcaaccca cgggggcctc tctggtcctg caggtgtcct acacgccacc
     1081 cccaggagct gtgcccctgt tcccaccacc tgcttctcta gcaccctccc cgacactgcc
     1141 tgacatggat ctggtgcctg gtgggggaca gagccgggct gagacttggt ccctgctcag
     1201 tgacagcacc atggacacga gatactctgg gaagaagtgg ccggtcccca cggacacagg
     1261 aggggaggaa gacaccgagg accagggcct gactggagat gaagctgagc cgttcctgga
     1321 ccagagtgct gctgtgggcc cagggggacc gaccacaccc aggaagccac cttcccaccc
     1381 tcctccccac tatccagggg caaaaagaaa aagaagctct gccccaccta gaaagctgct
     1441 gtcagacaag ccacaggact tccagatcag agtccaggtg atagaggggc gtcagctgcc
     1501 tggggtgaat attaagcctg tggtcaaggt cacagctgct gggcagacca agcgaactcg
     1561 gatccagaag ggaaatagcc cactcttcaa tgagactctt ttcttcaacg tatttgactc
     1621 tcccttggag ctgtttgatg agcccatctt catcacggtg gtggactcac gctcccttag
     1681 gacagatgcc ctccttggcg agttccggat ggatgtgggc accgtgtaca gagagccccg
     1741 acacgcttat ctccggaagt ggctgctgct ctccgaccct gatgacttct ctgcgggggc
     1801 cagaggctac ctcaaagcca gcctgtgtgt gctagggcct ggagatgaag ctcccctgga
     1861 caagaaggac ccttctgaag acaaagagga cattgaaggc aacctgctta ggcccaccgg
     1921 tgtggccctt cgaggagccc acttctgcct gaagctcttc cgggctgagg acttaccaca
     1981 gatggacgat gctgtgatgg acaacgtcaa gcagatcttt ggctttgaca gcaacaagaa
     2041 gaacttggtg gatccctttg tggaagtcag ctttgctggg aaaatgctct gcagcaagat
     2101 cctggagaag acagctaacc ctcagtggaa ccagaacatc accttgcctg cgatgtttcc
     2161 ctctatgtgt gaaaaaatga ggattcgtgt catggactgg gaccgcctca ctcacaatga
     2221 cactgtggcc accacctact tgggtatgtc gaaaatctct gctactggag gagaaataga
     2281 agtggacgac aacctgggct tcctccccac ttttgggccc tgctatgtga acctctatgg
     2341 cagtccccga gagttcacgg gcttcccaga tccttatgca gagctcaaca caggaaaggg
     2401 agaaggtgtg gcttatcgag gcagggttct gttgtccctg gagaccaaac tggtggaaca
     2461 cagtgagcag aaggtggaag accttcctgc tgatgatatc ctgcgtgtgg agaagtacct
     2521 gcggaggcgc aaatattccc tctttgctgc cttctactca gccacaatgc tgcaggatgt
     2581 ggatgacgcc atccagttcg aggtcagcat tgggaactac gggaacaagt tcgacaccac
     2641 ctgcctgccc ttggcctcca ccacccagta cagccgggcg gtctttgatg gatgccacta
     2701 ctattacttg ccttggggca acgtgaagcc cgtggtggtg ctgtcctcat actgggaaga
     2761 catcagccat cgaattgaga tccaaaacca gctcctcagg gtcgctgacc gcctggaagc
     2821 taacctggag caggtccact tggctctgaa ggcacagtgt tcctccgagg atgtggacgc
     2881 cctggtggct cagttgacag atgagctcct tgcagactgc agccagcccc tgtgtgacat
     2941 ccacgagata ccttctgcca cccaccttga tcagtacctg ctccggctac gcacccgtca
     3001 cctgagccag atcaaagaag ctgctctggc cttgaagctt ggccatagtg agctctccac
     3061 tgccctggag caggccgagg actggcttct gcatctccgt gccctggccg aggagcccca
     3121 gaacagcctg ccggacatca tcatctggat gctgcagggt gacaagcgtg tggcctacca
     3181 gcgggtgcct gcccatgaag tcctcttctc ccggcggggc cccagctact gcggcaggaa
     3241 ctgtgggaag ctgcagacca tctttctgaa atatcctatg gaaggtatgc ctggggcccg
     3301 aatgcccgtg cagattcgga tcaagctctg gttcgggctc tctgtggatg agaaggaatt
     3361 caatcagttt gctgagggaa agctctctgt ctttgccgaa acgtatgaga accagacaaa
     3421 gctggcactg gtcgggaact ggggcacaac gggcctcacc taccccaagt tttctgatgt
     3481 cacaggcaag atcaaactac caaaggatag cttccgcccc tcagctggct gggcctgggc
     3541 aggagattgg ttcgtgtgtc cagagaagac cctactccat gatgctgacg ctggtcatct
     3601 gagcttcgtg gaggaggtgt tcgagaacca gacccggctc cctgggggcc agtggattta
     3661 catgagtgat aactacacag atgtgaatgg ggagaaggta cttccgaagg atgatattga
     3721 atgcccgctg ggctggaagt gggaagatga agaatggtcc acagacctca atcgggctgt
     3781 tgatgaacaa ggctgggagt acagcatcac cataccccca gaccgaaagc caaaacactg
     3841 ggtccctgtt gagaagatgt attacactca caggcgtcga cgctgggtcc gactgcgcag
     3901 gagggacctc agccagatgg aagcactgaa gaggcacagg caggcagagg ccgagggcga
     3961 gggctgggag tatgcctctc tcttcggctg gaagttccac ctggagtacc gcaagacaga
     4021 tgccttccgc cgccgccgct ggcgccgccg catggagccg ctggagaaga cgggacctgc
     4081 agctgtgttt gccctcgagg gggccctggg cggcatggtg gatgacaaga gcgaagactc
     4141 catgtctgtt tctaccctga gctttggcgt taacagaccc acaatctcct gcatctttga
     4201 ctatgggaac cgctaccatc tgcgctgcta cctgtaccag gcccgagacc tgccagccat
     4261 ggacaaggac tccttctctg atccctacgc catcgtctcc ttcctgcatc aaagccagaa
     4321 gacagtggtg gagaagaaca ccctgaatcc cacctgggat caaaccctca tcttctatga
     4381 gattgagatc ttcggcgagc cagccagtat cgccgagcat ccgccctgca tcgtggtgga
     4441 gctctatgac cacgacacct atggtgctga cgagttcatg ggtcgctgta tttgtcagcc
     4501 aagcctggaa cggatgcccc gcctggcctg gttcccactg acccggggca gccagcctgc
     4561 cggcgagctg ctggcagcgt tcgagcttat ccagagagag aagccagcca tccatcacat
     4621 ccctggcttt gagatgcatg aaacttcaag gattttggat gagacggagg acacagattt
     4681 gccttaccca ccacctcaaa gggaggccaa catctacatg gttccccaga acattaagcc
     4741 agctctccaa cgtactgcca ttgagatcct ggcctggggc ctgcggaaca tgaagagcta
     4801 tcagatggcc agcatctctt cccccagcct cgtggtggag tgtggtgggc agacagtgca
     4861 gtcctgtgtc atcagaaacc ttcggaagaa ccccaacttt gatgtctgta ctctcttcat
     4921 ggaagtgatg ttacccagag aggacctcta ctgtccacct attgtggtca aggtcattga
     4981 taatcgccag tttggccgcc ggccagttgt gggtcagtgt accattcgct ccctggagaa
     5041 cttcctgtgc gacccatact cagcagagag tccatcccca cagggtggcc cagatgatgt
     5101 gagcttactc agccccgggg aagatgtgct gattgacatc gatgacaagg agcctctcat
     5161 tcctgtccag gaggaggagt tcattgactg gtggagcaaa ttctttgctt ctgtcgggga
     5221 gagggaaaag tgtggttcct acctggagaa ggattttgac accttgaagg tctatgacac
     5281 acagctggag aatgtggagg cttttggagg cctgtccgac ttttgtaaca ccttcaagct
     5341 ctaccggggc aggactcagg aggagacaga tgatccatct gtcatcggag aatttaaggg
     5401 tctcttcaaa atttatcccc tcccagaaga tccagccatc cccatgcccc cgagacaatt
     5461 ccatcagctg gctgctcagg gtccccagga atgcttggtc cgtatttata tcgtccgagc
     5521 atttggctta cagcccaagg accccaatgg caagtgtgac ccttacatca agatctccat
     5581 agggaagaaa tcagtgagtg accaggataa ctacatccct tgcaccctgg agcctgtatt
     5641 cggaaagatg tttgaactca cctgcactct acctctggag aaggacctga agatcacact
     5701 ctacgattat gacctgctct ccaaggatga gaagattggg gagacggtca tagacctgga
     5761 gaacaggctg ctgtccaagt ttggggctcg ctgtgggctc ccacagacct actgtgtctc
     5821 tggaccaaat cagtggagag atcagctccg cccctcccag ctcctccacc tcttctgcca
     5881 gcagcacagg atcaaggccc ccgtgtaccg gacagaccga gtgacgtttc aggataagga
     5941 ctacaccatt gaggagatag aggctggcag actcccaaac ccacacctgg gcccagtgga
     6001 ggaacgctta gccctgcatg tccttcagca acaaggcttg gttcctgagc atgtggagtc
     6061 acggcctctt tatagtcctc tgcagccaga tatcgagcag gggaagctac agatgtggat
     6121 tgacatattt ccaaaggtgc tgggccggcc tggacctccc ttcaacatca ccccacggaa
     6181 agctagaagg ttttttctgc gttgtattat ttggaacaca aaagatgtga tcttggatga
     6241 cctcagtctc acgggggaga agatgagtga catctatgtg aaaggctgga tggtgggatt
     6301 cgaagaacac aagcagaaga cagatgtgca ctaccgctcc ctgggtgggg agggcaattt
     6361 caactggagg ttcgtgtttc ccttcgacta tctgcctgct gagcaagtct gtgctgttgc
     6421 caagaaggat gccttctgga gactcgacaa gacagagagc aagatcccag cacgtgtggt
     6481 cttccagatc tgggacaatg acaaattctc ttttgatgac tttctgggct ctctgcagct
     6541 ggatctcaac cgaatgccca agccagccaa gacggctgag aagtgctcct tggaccagct
     6601 ggatgacacc ttccacccag aatggtttgt gtcccttttt gagcagaaga cagtgaaagg
     6661 atggtggcct tgtgtgacag aggagggcga gaagaagatg ttggcgggca agctggaaat
     6721 gaccttggag attgttgcag aaagtgaaca tgaagagcgg cctgctggcc aaggtcggga
     6781 tgaacccaac atgaatccga agctagagga tccaaggcgc cccgatactt ctttcctgtg
     6841 gttcacctcc ccgtacaaga ccatgaagtt catcttgtgg cgacgcttcc ggtgtgccat
     6901 catcctcttc atcatcctct tcatcctcct gctcttcctg ggtgtctttg tctacgcctt
     6961 cccgaactac gctgccatga agctggtgaa gccctttcga tgagcatatg ttaattaacc
     7021 taggttataa gcggccgcac ccagctttct tgtacaaagt tggcattata agaaagcatt
     7081 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     7141 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     7201 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata atatcatcat
     7261 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     7321 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     7381 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     7441 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     7501 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     7561 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     7621 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     7681 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     7741 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     7801 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     7861 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     7921 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     7981 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     8041 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     8101 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     8161 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     8221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8881 tcctggcctt ttgctggcct tttgctcaca tgtt
//
