LOCUS       sequence-96324-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK312
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(8..32)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(565..584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagtaaacc cagctttctt gtacaaagtg gtggcgcgcc tatgttacgt gcaaaggtga
       61 ttgtcacccc ccgaaagacc atattgtgac acaccctcag tatcacgccc aaacatttac
      121 agccgcggtg tcaaaaaccg cgtggacgtg gttaacatcc ctgctgggag gatcagccgt
      181 aattattata attggcttgg tgctggctac tattgtggcc atgtacgtgc tgaccaacca
      241 gaaacataat tgaatacagc agcaattggc aagctgctta catagaactc gcggcgattg
      301 gcatgccgcc ttaaaatttt tattttattt ttcttttctt ttccgaatcg gattttgttt
      361 ttaatatttc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tagggataac
      421 agggtaattg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      781 gtaactatcg tcttgagtcc aacccgg
//
