LOCUS       sequence-96327-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in 
            neurons in zebrafish larva. The plasmid is in Tol2 transposon 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Freeman J, Vladimirov N, Kawashima T, Mu Y, Sofroniew NJ, Bennett 
            DV, Rosen J, Yang CT, Looger LL, Ahrens MB
  TITLE     Mapping brain activity at scale with cluster computing.
  JOURNAL   Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041.
  PUBMED    25068736
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Jul 27. doi: 10.1038/nmeth.3041."
COMMENT     Sequence Label: Tol2-elavl3-H2B-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     589..608
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 acagtaaaac gacggccagg atccaccggt cacttcgctg tcatcatttg tacaaactct
       61 tcgtagttta cctgaccatc cccatcgatg tctgcttccc tgatcatttc atcaacctct
      121 tcatctgtta acttctctcc aaggtttgtc atcacgtggc gaagctctgc tgcactgatg
      181 tagccattgc catccttatc aaacacaccg aacgcttctc taatttcttc ttccgtgtcc
      241 ctgtatttca tttttcttgc catcattgtc aggaactcag ggaagtcgat tgtgccgtca
      301 ccgtcggcat ctacttcatt gatcatgtcc tgcagctctg cttctgtggg gttctgcccc
      361 agagaccgca tcaccgtccc cagctccttg gttgttattg tcccatcccc gtccttgtca
      421 aatagggaga aagcctcttt aaattctgcg atctgctctt cagtcagttg gtccggcagg
      481 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      541 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      601 ccgtcgtcct tgaagaagat ggtgcgctcc tggatgtagc cttcgggcat ggcggacttg
      661 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
      721 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
      781 agggtcagct tgccgtaggt ggcatcgccc tcaccctcgc cggacacgct gaacttgtgg
      841 ccgtt
//
