LOCUS       sequence-96330-e        8857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human dysferlin (accession #NM_003494) cDNA in pDONR221 plasmid 
            which can be used as an entry vector for Invitrogen's gateway 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8857)
  TITLE     Human dysferlin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Human-Dysferlin-Original
FEATURES             Location/Qualifiers
     source          1..8857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1382..1393
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6982..7081)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(7098..7117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7099..7117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7122..7138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7240..7259)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7251..8060
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7318..7337)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8207..8795
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8696..8715
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctca ctagtggtac cgtttaaacg atcggccgcc accatgctga gggtcttcat
      721 cctctatgcc gagaacgtcc acacacccga caccgacatc agcgatgcct actgctccgc
      781 ggtgtttgca ggggtgaaga agagaaccaa agtcatcaag aacagcgtga accctgtatg
      841 gaatgaggga tttgaatggg acctcaaggg catccccctg gaccagggct ctgagcttca
      901 tgtggtggtc aaagaccatg agacgatggg gaggaacagg ttcctggggg aagccaaggt
      961 cccactccga gaggtcctcg ccacccctag tctgtccgcc agcttcaatg cccccctgct
     1021 ggacaccaag aagcagccca caggggcctc gctggtcctg caggtgtcct acacaccgct
     1081 gcctggagct gtgcccctgt tcccgccccc tactcctctg gagccctccc cgactctgcc
     1141 tgacctggat gtagtggcag acacaggagg agaggaagac acagaggacc agggactcac
     1201 tggagatgag gcggagccat tcctggatca aagcggaggc ccgggggctc ccaccacccc
     1261 aaggaaacta ccttcacgtc ctccgcccca ctaccccggg atcaaaagaa agcgaagtgc
     1321 gcctacatct agaaagctgc tgtcagacaa accgcaggat ttccagatca gggtccaggt
     1381 gatcgagggg cgccagctgc cgggggtgaa catcaagcct gtggtcaagg ttaccgctgc
     1441 agggcagacc aagcggacgc ggatccacaa gggaaacagc ccactcttca atgagactct
     1501 tttcttcaac ttgtttgact ctcctgggga gctgtttgat gagcccatct ttatcacggt
     1561 ggtagactct cgttctctca ggacagatgc tctcctcggg gagttccgga tggacgtggg
     1621 caccatttac agagagcccc ggcacgccta tctcaggaag tggctgctgc tctcagaccc
     1681 tgatgacttc tctgctgggg ccagaggcta cctgaaaaca agcctttgtg tgctggggcc
     1741 tggggacgaa gcgcctctgg agagaaaaga cccctctgaa gacaaggagg acattgaaag
     1801 caacctgctc cggcccacag gcgtagccct gcgaggagcc cacttctgcc tgaaggtctt
     1861 ccgggccgag gacttgccgc agatggacga tgccgtgatg gacaacgtga aacagatctt
     1921 tggcttcgag agtaacaaga agaacttggt ggaccccttt gtggaggtca gctttgcggg
     1981 gaaaatgctg tgcagcaaga tcttggagaa gacggccaac cctcagtgga accagaacat
     2041 cacactgcct gccatgtttc cctccatgtg cgaaaaaatg aggattcgta tcatagactg
     2101 ggaccgcctg actcacaatg acatcgtggc taccacctac ctgagtatgt cgaaaatctc
     2161 tgcccctgga ggagaaatag aagaggagcc tgcaggtgct gtcaagcctt cgaaagcctc
     2221 agacttggat gactacctgg gcttcctccc cacttttggg ccctgctaca tcaacctcta
     2281 tggcagtccc agagagttca caggcttccc agacccctac acagagctca acacaggcaa
     2341 gggggaaggt gtggcttatc gtggccggct tctgctctcc ctggagacca agctggtgga
     2401 gcacagtgaa cagaaggtgg aggaccttcc tgcggatgac atcctccggg tggagaagta
     2461 ccttaggagg cgcaagtact ccctgtttgc ggccttctac tcagccacca tgctgcagga
     2521 tgtggatgat gccatccagt ttgaggtcag catcgggaac tacgggaaca agttcgacat
     2581 gacctgcctg ccgctggcct ccaccactca gtacagccgt gcagtctttg acgggtgcca
     2641 ctactactac ctaccctggg gtaacgtgaa acctgtggtg gtgctgtcat cctactggga
     2701 ggacatcagc catagaatcg agactcagaa ccagctgctt gggattgctg accggctgga
     2761 agctggcctg gagcaggtcc acctggccct gaaggcgcag tgctccacgg aggacgtgga
     2821 ctcgctggtg gctcagctga cggatgagct catcgcaggc tgcagccagc ctctgggtga
     2881 catccatgag acaccctctg ccacccacct ggaccagtac ctgtaccagc tgcgcaccca
     2941 tcacctgagc caaatcactg aggctgccct ggccctgaag ctcggccaca gtgagctccc
     3001 tgcagctctg gagcaggcgg aggactggct cctgcgtctg cgtgccctgg cagaggagcc
     3061 ccagaacagc ctgccggaca tcgtcatctg gatgctgcag ggagacaagc gtgtggcata
     3121 ccagcgggtg cccgcccacc aagtcctctt ctcccggcgg ggtgccaact actgtggcaa
     3181 gaattgtggg aagctacaga caatctttct gaaatatccg atggagaagg tgcctggcgc
     3241 ccggatgcca gtgcagatac gggtcaagct gtggtttggg ctctctgtgg atgagaagga
     3301 gttcaaccag tttgctgagg ggaagctgtc tgtctttgct gaaacctatg agaacgagac
     3361 taagttggcc cttgttggga actggggcac aacgggcctc acctacccca agttttctga
     3421 cgtcacgggc aagatcaagc tacccaagga cagcttccgc ccctcggccg gctggacctg
     3481 ggctggagat tggttcgtgt gtccggagaa gactctgctc catgacatgg acgccggtca
     3541 cctgagcttc gtggaagagg tgtttgagaa ccagacccgg cttcccggag gccagtggat
     3601 ctacatgagt gacaactaca ccgatgtgaa cggggagaag gtgcttccca aggatgacat
     3661 tgagtgccca ctgggctgga agtgggaaga tgaggaatgg tccacagacc tcaaccgggc
     3721 tgtcgatgag caaggctggg agtatagcat caccatcccc ccggagcgga agccgaagca
     3781 ctgggtccct gctgagaaga tgtactacac acaccgacgg cggcgctggg tgcgcctgcg
     3841 caggagggat ctcagccaaa tggaagcact gaaaaggcac aggcaggcgg aggcggaggg
     3901 cgagggctgg gagtacgcct ctctttttgg ctggaagttc cacctcgagt accgcaagac
     3961 agatgccttc cgccgccgcc gctggcgccg tcgcatggag ccactggaga agacggggcc
     4021 tgcagctgtg tttgcccttg agggggccct gggcggcgtg atggatgaca agagtgaaga
     4081 ttccatgtcc gtctccacct tgagcttcgg tgtgaacaga cccacgattt cctgcatatt
     4141 cgactatggg aaccgctacc atctacgctg ctacatgtac caggcccggg acctggctgc
     4201 gatggacaag gactcttttt ctgatcccta tgccatcgtc tccttcctgc accagagcca
     4261 gaagacggtg gtggtgaaga acacccttaa ccccacctgg gaccagacgc tcatcttcta
     4321 cgagatcgag atctttggcg agccggccac agttgctgag caaccgccca gcattgtggt
     4381 ggagctgtac gaccatgaca cttatggtgc agacgagttt atgggtcgct gcatctgtca
     4441 accgagtctg gaacggatgc cacggctggc ctggttccca ctgacgaggg gcagccagcc
     4501 gtcgggggag ctgctggcct cttttgagct catccagaga gagaagccgg ccatccacca
     4561 tattcctggt tttgaggtgc aggagacatc aaggatcctg gatgagtctg aggacacaga
     4621 cctgccctac ccaccacccc agagggaggc caacatctac atggttcctc agaacatcaa
     4681 gccagcgctc cagcgtaccg ccatcgagat cctggcatgg ggcctgcgga acatgaagag
     4741 ttaccagctg gccaacatct cctcccccag cctcgtggta gagtgtgggg gccagacggt
     4801 gcagtcctgt gtcatcagga acctccggaa gaaccccaac tttgacatct gcaccctctt
     4861 catggaagtg atgctgccca gggaggagct ctactgcccc cccatcaccg tcaaggtcat
     4921 cgataaccgc cagtttggcc gccggcctgt ggtgggccag tgtaccatcc gctccctgga
     4981 gagcttcctg tgtgacccct actcggcgga gagtccatcc ccacagggtg gcccagacga
     5041 tgtgagccta ctcagtcctg gggaagacgt gctcatcgac attgatgaca aggagcccct
     5101 catccccatc caggaggaag agttcatcga ttggtggagc aaattctttg cctccatagg
     5161 ggagagggaa aagtgcggct cctacctgga gaaggatttt gacaccctga aggtctatga
     5221 cacacagctg gagaatgtgg aggcctttga gggcctgtct gacttttgta acaccttcaa
     5281 gctgtaccgg ggcaagacgc aggaggagac agaagatcca tctgtgattg gtgaatttaa
     5341 gggcctcttc aaaatttatc ccctcccaga agacccagcc atccccatgc ccccaagaca
     5401 gttccaccag ctggccgccc agggacccca ggagtgcttg gtccgtatct acattgtccg
     5461 agcatttggc ctgcagccca aggaccccaa tggaaagtgt gatccttaca tcaagatctc
     5521 catagggaag aaatcagtga gtgaccagga taactacatc ccctgcacgc tggagcccgt
     5581 atttggaaag atgttcgagc tgacctgcac tctgcctctg gagaaggacc taaagatcac
     5641 tctctatgac tatgacctcc tctccaagga cgaaaagatc ggtgagacgg tcgtcgacct
     5701 ggagaacagg ctgctgtcca agtttggggc tcgctgtgga ctcccacaga cctactgtgt
     5761 ctctggaccg aaccagtggc gggaccagct ccgcccctcc cagctcctcc acctcttctg
     5821 ccagcagcat agagtcaagg cacctgtgta ccggacagac cgtgtaatgt ttcaggataa
     5881 agaatattcc attgaagaga tagaggctgg caggatccca aacccacacc tgggcccagt
     5941 ggaggagcgt ctggctctgc atgtgcttca gcagcagggc ctggtcccgg agcacgtgga
     6001 gtcacggccc ctctacagcc ccctgcagcc agacatcgag caggggaagc tgcagatgtg
     6061 ggtcgaccta tttccgaagg ccctggggcg gcctggacct cccttcaaca tcaccccacg
     6121 gagagccaga aggtttttcc tgcgttgtat tatctggaat accagagatg tgatcctgga
     6181 tgacctgagc ctcacggggg agaagatgag cgacatttat gtgaaaggtt ggatgattgg
     6241 ctttgaagaa cacaagcaaa agacagacgt gcattatcgt tccctgggag gtgaaggcaa
     6301 cttcaactgg aggttcattt tccccttcga ctacctgcca gctgagcaag tctgtaccat
     6361 tgccaagaag gatgccttct ggaggctgga caagactgag agcaaaatcc cagcacgagt
     6421 ggtgttccag atctgggaca atgacaagtt ctcctttgat gattttctgg gctccctgca
     6481 gctcgatctc aaccgcatgc ccaagccagc caagacagcc aagaagtgct ccttggacca
     6541 gctggatgat gctttccacc cagaatggtt tgtgtccctt tttgagcaga aaacagtgaa
     6601 gggctggtgg ccctgtgtag cagaagaggg tgagaagaaa atactggcgg gcaagctgga
     6661 aatgaccttg gagattgtag cagagagtga gcatgaggag cggcctgctg gccagggccg
     6721 ggatgagccc aacatgaacc ctaagcttga ggacccaagg cgccccgaca cctccttcct
     6781 gtggtttacc tccccataca agaccatgaa gttcatcctg tggcggcgtt tccggtgggc
     6841 catcatcctc ttcatcatcc tcttcatcct gctgctgttc ctggccatct tcatctacgc
     6901 cttcccgaac tatgctgcca tgaagctggt gaagcccttc agctgagcat atgttaatta
     6961 acctaggtta taagcggccg cacccagctt tcttgtacaa agttggcatt ataagaaagc
     7021 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     7081 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     7141 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat
     7201 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
     7261 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     7321 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
     7381 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     7441 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
     7501 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
     7561 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     7621 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     7681 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     7741 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     7801 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     7861 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     7921 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     7981 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     8041 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     8101 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     8161 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8221 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8281 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8341 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8401 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8461 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8521 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8581 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8641 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8701 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8761 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8821 ggttcctggc cttttgctgg ccttttgctc acatgtt
//
