LOCUS       sequence-96332-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK095
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..70)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccttgctcac cattgggcct gggttctcct ccacgtcgcc ggcctgcttc agcagagaga
       61 agttggtggc gccagatccc ttctgaaggc ctttaatctt ctccacaagg cttccaagct
      121 cctttctcag ctctccctcg ttgattatcg ctgccgaagc gcatggcacc tcaatgccca
      181 cagcccttcc aaggtcgttc ttgcttttaa cgtaaatgta cggcacattc ttctcctcgc
      241 agaggagggg cagatgagca acgatctcag gcgggtcaac atcctctgcg atgtaaacga
      301 gctttgccag tcccctctcc acagcctttg tcgtctcgtt ggtacctttc tttaccttac
      361 cgctctccct aaccttctcc agcagactca gagcttcgtt ctgcatgtcc tcaggaacct
      421 caaatctcac gtacatggtg gcactatgct gactatttag gaccaccgta gagatgcttt
      481 atttcccctc tgatggcttg gaatgctctt ttgctctggg caaaagttct caatgccagt
      541 aggacaggtg taatattgtc tacctcatac cgggtcgtca cggccactgc taaagtgcct
      601 gttataccta ctctaaacca cgcctttgtt tcatctagca gagcgcgtct tctgtcttcg
      661 tcttgctcgt cgtcggctgg gagcggttta cccaacttaa acagcctttt caggggatcc
      721 gccacgcggc acgctgtgga agtaaccgaa tcttgcaaga taaatccgcc gcagaagtaa
      781 ggtggtctct caccgatgac tgcgtcgatg atcttaacct ccatgttgag ccaggtggcg
      841 cacctctcag ccatttc
//
