LOCUS       sequence-96335-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6-L469.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ381
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(9..60)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     promoter        complement(165..521)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(558..574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(558..574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(571..593)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    582..598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(606..612,613..630,631..636))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    651..672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(789..806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acggtacccc tgtttcgtcc tcacggactc atcagaccgg aaagcacatc cggtgacagg
       61 gcaccaccga ctcggtgcca ctcacttttt tcaagttgat aacggactag ccttatttta
      121 acttgctatt tctagctcta aaaccaaggc atctgaacaa tagattcttc ggtacaggtt
      181 atgttttttg gcaacagcaa ctattcaaat caactgaaag ttttttagaa ttcaacaact
      241 tccaatactc aagtgtccaa gctagcaaac tgtttaataa tcgtgaccca acattccttt
      301 catgcgcacc ctcagcgtca cataaatcaa cactttacaa cacctattca aagcttctat
      361 atgccttcgt catttaaatg tctaacattt caaaaaagtg tttgctaatg gtaaattctt
      421 gagttttctt ggaagcacat atataacgag tgtgactgtt ctacgctcct tgctgactag
      481 tcatcagtct acgcatgcca actaccacca acataagcaa aaggtacccg gggtaccgag
      541 ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      601 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      661 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      721 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      841 gctcactc
//
