LOCUS       sequence-96338-e         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6-L469.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ381
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
       61 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
      121 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat
      181 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
      241 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
      301 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
      361 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
      421 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
      481 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtgaagac
      541 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat
      601 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga
      661 ttgtctttcc agactgctta tctcggatcc cattgatcag
//
