LOCUS       sequence-96342-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: Empty sgRNA target (CspCI placeholder) (see 
            comments)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ374
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcgaacttc cgttgtgtga tcagtttggc gttcagcagc tgccgccaat aatttttcat
       61 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct
      121 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc
      181 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc
      241 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg
      301 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc
      361 cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat
      421 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg
      481 cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtgaagact
      541 gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc
      601 atggatcaac tgcatgaagt tccggttggc aaatccatcg gacttaagaa aatccaggat
      661 tgtctttcca gactgcttat ctcggatccc attgatcagt tttcttgaca gccg
//
