LOCUS       sequence-96344-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: Empty sgRNA target (CspCI placeholder) (see 
            comments)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ374
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc
       61 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg
      121 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc
      181 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat
      241 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc
      301 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca
      361 cgaacagctg cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg
      421 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct
      481 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg
      541 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga
      601 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc
      661 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga
      721 ctgtaggaga atcgaatccg ccgtatttct t
//
