LOCUS       sequence-96346-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ288
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(155..511)
                     /label=rrk1 promoter/leader
                     /note="promoter and leader RNA for the Schizosaccharomyces 
                     pombe rrk1 gene encoding the K-RNA component of RNase P 
                     (Jacobs et al., 2014)"
     primer_bind     complement(548..564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(561..583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    572..588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(596..602,603..620,621..626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    641..662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtttcgtcc tcacggactc atcagaccgg aaagcacatc cggtgacagg gcaccaccga
       61 ctcggtgcca ctcacttttt tcaagttgat aacggactag ccttatttta acttgctatt
      121 tctagctcta aaaccgaggc atctgaacaa tagattcttc ggtacaggtt atgttttttg
      181 gcaacagcaa ctattcaaat caactgaaag ttttttagaa ttcaacaact tccaatactc
      241 aagtgtccaa gctagcaaac tgtttaataa tcgtgaccca acattccttt catgcgcacc
      301 ctcagcgtca cataaatcaa cactttacaa cacctattca aagcttctat atgccttcgt
      361 catttaaatg tctaacattt caaaaaagtg tttgctaatg gtaaattctt gagttttctt
      421 ggaagcacat atataacgag tgtgactgtt ctacgctcct tgctgactag tcatcagtct
      481 acgcatgcca actaccacca acataagcaa aaggtacccg gggtaccgag ctcgaattcg
      541 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      721 aatgaatcgg ccaacgcgc
//
