LOCUS       sequence-96347-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6+.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ288
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..23)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cacaccttcc tcttcttctt ggggtcagcc ctgctgtctc caccgagctg agagaggtcg
       61 attcttgttt catagagccc cgtaattgac tgatgaatca gtgtggcgtc caggacctcc
      121 tttgtagagg tgtaccgctt tctgtctatg gtggtgtcga agtacttgaa ggctgcaggc
      181 gcgcccaagt tggtcagagt aaacaagtgg ataatgtttt ctgcctgctc cctgatgggc
      241 ttatccctgt gcttattgta agcagaaagc accttatcga ggttagcgtc ggcgaggatc
      301 actcttttgg agaattcgct tatttgctcg atgatctcat caaggtagtg tttgtgttgt
      361 tccacgaaca gctgcttctg ctcattatct tcgggagacc ctttgagctt ttcatagtgg
      421 ctggccagat acaagaaatt aacgtattta gagggcagtg ccagctcgtt acctttctgc
      481 agctcgcccg cactagcgag cattcgtttc cggccgtttt caagctcaaa gagagagtac
      541 ttgggaagct taatgatgag gtcttttttg acctctttat atcctttcgc ctcgagaaag
      601 tcgatggggt ttttttcgaa gcttgatcgc tccatgattg tgatgcccag cagttccttg
      661 acgcttttga gttttttaga cttccctttc tccactttgg ccacaaccag tacactgtaa
      721 gcgactgtag gagaatcgaa tccgccgtat ttctt
//
