LOCUS       sequence-96350-e         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6-M210.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ289
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(94..105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgaacttccg ttgtgtgatc agtttggcgt tcagcagctg ccgccaataa tttttcattt
       61 tcttgacaac ttcttctgag gggacgttat cactcttccc tctattttta tcggatcttg
      121 tcaacacttt attatcaata gaatcatctt tgagaaaaga ctggggcacg atatgatcca
      181 cgtcgtagtc ggagagccga ttgatgtcca gttcctgatc cacgtacatg tccctgccgt
      241 tctgcaggta gtacaggtag agcttctcat tctgaagctg ggtgttttca actgggtgtt
      301 ccttaaggat ttgggacccc agttctttta taccctcttc aatcctcttc atcctttccc
      361 tactgttctt ctgtcccttc tgggtagttt ggttctctcg ggccatctcg ataacgatat
      421 tctcgggctt atgccttccc attactttga cgagttcatc cacgacctta acggtctgca
      481 gtattccctt tttgatagct gggctacctg caagattagc gatgtgctcg tgaagactgt
      541 ccccctggcc agaaacttgt gctttctgga tgtcctcctt aaaggtgaga gagtcatcat
      601 ggatcaactg catgaagttc cggttggc
//
