LOCUS       sequence-96351-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in 
            fission yeast: sgRNA targeted against ade6-M210.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Jacobs JZ, Ciccaglione KM, Tournier V, Zaratiegui M
  TITLE     Implementation of the CRISPR-Cas9 system in fission yeast.
  JOURNAL   Nat Commun. 2014 Oct 29;5:5344. doi: 10.1038/ncomms6344.
  PUBMED    25352017
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-29"; volume: "5:5344. doi"; pages: " 
            10.1038/ncomms6344"
COMMENT     Sequence Label: pMZ289
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..24)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acacaccttc ctcttcttct tggggtcagc cctgctgtct ccaccgagct gagagaggtc
       61 gattcttgtt tcatagagcc ccgtaattga ctgatgaatc agtgtggcgt ccaggacctc
      121 ctttgtagag gtgtaccgct ttctgtctat ggtggtgtcg aagtacttga aggctgcagg
      181 cgcgcccaag ttggtcagag taaacaagtg gataatgttt tctgcctgct ccctgatggg
      241 cttatccctg tgcttattgt aagcagaaag caccttatcg aggttagcgt cggcgaggat
      301 cactcttttg gagaattcgc ttatttgctc gatgatctca tcaaggtagt gtttgtgttg
      361 ttccacgaac agctgcttct gctcattatc ttcgggagac cctttgagct tttcatagtg
      421 gctggccaga tacaagaaat taacgtattt agagggcagt gccagctcgt tacctttctg
      481 cagctcgccc gcactagcga gcattcgttt ccggccgttt tcaagctcaa agagagagta
      541 cttgggaagc ttaatgatga ggtctttttt gacctcttta tatcctttcg cctcgagaaa
      601 gtcgatgggg tttttttcga agcttgatcg ctccatgatt gtgatgccca gcagttcctt
      661 gacgcttttg agttttttag acttcccttt ctccactttg gccacaacca gtacactgta
      721 agcgactgta ggagaatcga atccgccgta tttctt
//
