LOCUS       sequence-96353-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of GFP hBub1 K821R in the genome and 
            Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Perera D, Taylor SS
  TITLE     Sgo1 establishes the centromeric cohesion protection mechanism in G2
            before subsequent Bub1-dependent recruitment in mitosis.
  JOURNAL   J Cell Sci. 2010 Mar 1;123(Pt 5):653-9. doi: 10.1242/jcs.059501. 
            Epub 2010 Feb 2.
  PUBMED    20124418
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2010-03-1"; volume: "123(Pt 5):653-9. doi"; pages: " 
            10.1242/jcs.059501. Epub 2010 Feb 2"
COMMENT     Sequence Label: pcDNA5 FRT TO GFP hBub1 K821R
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acgagctgta caagctcgag ggatccgaca ccccggaaaa tgtccttcag atgcttgaag
       61 cccacatgca gagctacaag ggcaatgacc ctcttggtga atgggaaaga tacatacagt
      121 gggtagaaga gaattttcct gagaataaag aatacttgat aactttacta gaacatttaa
      181 tgaaggaatt tttagataag aagaaatacc acaatgaccc aagattcatc agttattgtt
      241 taaaatttgc tgagtacaac agtgacctcc atcaattttt tgagtttctg tacaaccatg
      301 ggattggaac cctgtcatcc cctctgtaca ttgcctgggc ggggcatctg gaagcccaag
      361 gagagctgca gcatgccagt gctgtccttc agagaggaat tcaaaaccag gctgaaccca
      421 gagagttcct gcaacaacaa tacaggttat ttcagacacg cctcactgaa acccatttgc
      481 cagctcaagc tagaacctca gaacctctgc ataatgttca ggttttaaat caaatgataa
      541 catcaaaatc aaatccagga aataacatgg cctgcatttc taagaatcag ggttcagagc
      601 tttctggagt gatatcttca gcttgtgata aagagtcaaa tatggaacga agagtgatca
      661 cgatttctaa atcagaatat tctgtgcact catctttggc atccaaagtt gatgttgagc
      721 aggttgttat gtattgcaag gagaagctta ttcgt
//
