LOCUS       sequence-96354-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI 
            mutated)-P2A-EBFP2 4xFF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK333
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(421..439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        507..611
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgggatcac tctcggcatg gacgagctgt acaagtaaac tagtaaaagc actctgattt
       61 gacaattaaa gcactctgat ttgacaatta aagcactctg atttgacaat taaagcactc
      121 tgatttgaca attaaagcgg ccgcgactct agatcatact cgaggaagcg cagtacataa
      181 tgctgcgcag tgttgccaca taaccactat attaaccatt tatctagcgg acgccaaaaa
      241 ctcaatgtat ttctgaggaa gcgtggtgca taatgccacg cagcgtctgc ataactttta
      301 ttatttcttt tattaatcaa caaaattttg tttttaacat ttcaaaaaaa aaaaaaaaaa
      361 aaaaaaaaaa aaaaaaaaag gggaattcga gctcgggaat taattcttga agacgaaagg
      421 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      481 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      541 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      601 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      661 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      721 agttgggtgc acgagtgggt tacatcgaac tggatctca
//
