LOCUS       sequence-96363-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector for Chrimson-tdTomato in a floxed/forward-reading,
            Cre-dependent manner (Cre turns gene off).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pCAG-FLEX-fwd[Chrimson-tdT]
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    58..91
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    154..187
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      201..210
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcccata
       61 acttcgtata aagtatccta tacgaagtta tatcaaaata ggaagaccaa tgcttcacca
      121 tcgacccgaa ttgccaagca tcaccatcga cccataactt cgtataatgt atgctatacg
      181 aagttatact agctagcgcc gccaccatgg ctgagctgat cagcagcgcc accagatctc
      241 tgtttgccgc cggaggcatc aacccttggc ctaaccccta ccaccacgag gacatgggct
      301 gtggaggaat gacacctaca ggcgagtgct tcagcaccga gtggtggtgt gacccttctt
      361 acggactgag cgacgccgga tacggatatt gcttcgtgga ggccacaggc ggctacctgg
      421 tcgtgggagt ggagaagaag caggcttggc tgcacagcag aggcacacca ggagaaaaga
      481 tcggcgccca ggtctgccag tggattgctt tcagcatcgc catcgccctg ctgacattct
      541 acggcttcag cgcctggaag gccacttgcg gttgggagga ggtctacgtc tgttgcgtcg
      601 aggtgctgtt cgtgaccctg gagatcttca aggagttcag cagccccgcc acagtgtacc
      661 tgtctaccgg caaccacgcc tattgcctgc gctacttcga gtggctgctg tcttgccccg
      721 tgatcctgat caagctgagc aacctgagcg gcctgaagaa cgactacagc aagcggacca
      781 tgggcctgat cgtgtcttgc gtgggaatga tcgtgttcgg catggccgca ggactggcta
      841 ccgattggct caagtggctg ctgtatatcg tgtcttgcat ctacggcggc tacatgtact
      901 tccaggccgc caagtgctac gtggaagcca accacagcgt gcctaaaggc cattgccgca
      961 tggtcgtgaa gctgatggcc tacgcttact tcgcctcttg gggcagctac ccaatcctct
     1021 gggcagtggg accagaagga ctgctgaagc tgag
//
