LOCUS       sequence-96378-e       10802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse NAIP2 expressed under the constitutive, moderate-level 
            retroviral LTR promoter, expresses in mammalian cells; IRES-GFP 
            reporter downstream of NAIP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Kofoed EM, Vance RE
  TITLE     Innate immune recognition of bacterial ligands by NAIPs determines 
            inflammasome specificity.
  JOURNAL   Nature. 2011 Aug 28;477(7366):592-5. doi: 10.1038/nature10394.
  PUBMED    21874021
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-08-28- 28"; volume: "477"; issue: "7366"; 
            pages: "592-5"
COMMENT     Sequence Label: mscv2.2-NAIP2
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..79
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     95..111
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4055..4066)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    4537..5103
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4553..5105
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4697..4714)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4924..4943
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5117..5119,5120..5122,5123..5836)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5162..5183)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5423..5442)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5770..5791
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5889..6403
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6803..6820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6974..7562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7054..7073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7733..8524,8525..8593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8356..8375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8594..8698)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8766..8784
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8822..8844)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8944..8963
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9157..9179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9307..9326
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9526..10042
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    10106..10447
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(10514..10802,1..127)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgcccgt ctctccccct
       61 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
      121 aggcgccgga attagatccg ccaccatggc agcccaggga gaagccgttg aggagataat
      181 ttgtgagttt gatgacgact tggtctctga gctctctact cttctcagag tggatgcact
      241 ttcagtcttg aagagacaac aagaagaaga ccataaaaca cgaatgaaaa tgaagaaagg
      301 ttttaactca cagatgcgca gtgaagccaa acgactaaag acttttgaga cctatgacaa
      361 gttcagatca tggacgccac aggagatggc agctgctggg ttttaccaca caggggtgaa
      421 acttggggtt cagtgctttt gctgtagctt gatcctcttt tccaccagac tcaggaaact
      481 tcccatagag aaccacaaga aattacgacc agaatgtgag ttccttctgg gcaaagatgt
      541 tggtaacatt ggcaagtatg acatccgggt taagagtcca gagaagatgc tgagaggtga
      601 caaagcaagg taccatgaag aggaggcaag actggagtcc tttgaggact ggccatttta
      661 tgcccatggg acatcaccac gtgtactctc agcagctggc tttgtcttta caggtaaaag
      721 ggacactgtg cagtgtttct cctgtggtgg atgcttgggc aactgggaag aaggagatga
      781 tccctggaag gagcatgcca agtggttccc caaatgtgaa tttcttcaaa gtaagaagtc
      841 cccagaggaa attacccaat atgtacaaag ctatgaagga tttcttcatg taacgggaga
      901 acattttgtg aattcctggg tcagaagaga attacctatg gtatcagctt actgcaatga
      961 cagcgtcttc gctaatgaag aactaaggat ggacacgttt aaggactggc cccacgaatc
     1021 acccggggct gttgaagctc tggtcaaagc aggccttttc tacacaggca aaagggacat
     1081 tgtccagtgt ttttcctgtg gaggatgtat ggagaagtgg gcagaaggtg acaacccaat
     1141 agaagatcac accaagtttt ttcccaactg tgtgtttctc caaaccctga agtcctctgc
     1201 agaagtgatt ccagcccttc agagccactg tgcacttcca gaagccatgg aaaccacaag
     1261 tgaaagcaac catgatgatg cagcagcagt tcattctaca gtggtggatg tttctccaag
     1321 tgaagcccag gaacttgaac ctgcatccag cttggtgtct gttctctgta gagaccagga
     1381 ccacagtgaa gcccaagggc gtggatgtgc atccagtggg acctatcttc cctccacaga
     1441 cttgggtcag agtgaagccc agtggcttca agaggccagg agtctgagtg agcagctgag
     1501 agacacctac accaaagcta ctttccgcca catgaacttg ccagaagtgt actccagcct
     1561 cggcactgac catttgctca gctgcgatgt gtccatcatt tcaaagcaca tcagccagcc
     1621 agtgcaaggg tccctgacga tccctgaggt cttctccaat ctcaactctg tcatgtgtgt
     1681 ggagggggaa gctggcagtg gaaagacaac cttcctgaag aggatagctt ttctctgggc
     1741 atcaggatgc tgccccctgt tgaacaggtt ccagctggtc ttctacctct cccttagttc
     1801 catcacacca ggccaggaac tggccaaaat catctgtgcc caactcctag gggcaggagg
     1861 ctgcattagt gaagtgtgtc tgagcagcat catccagcag ttacaacacc aggtgctgtt
     1921 cctgttggat gactacagtg ggctggcctc actcccccaa gccctacaca cactgattac
     1981 aaaaaactac ttgtcacgga cctgcttatt gatcgctgtg catacaaaca aggtcagggg
     2041 catccgccca tacctagata cgagtctaga gatcaaagag tttcccttct ataatactgt
     2101 ctctgtgtta cggaagcttt tttcacatga cataatgcgt gtacgaaaat ttataaatta
     2161 ctttggattt catgaagagt tgcagggaat tcacaagaca cccctcttcg tggcagcagt
     2221 atgtactgac tggtttaaaa atccatctga ccagcccttt caggatgtgg cacttttcaa
     2281 ggcctatatg caatacttgt ccttaaagca caaaggtgca gctaagcctc tccaggccac
     2341 agtgtcctca tgtgggcagc tggccttgac agggcttttc tcatcgtgct ttgagttcaa
     2401 tagtgataac ctggcagagg caggagttga tgaagatgaa gagctcacca cctgcttgat
     2461 gagcaaattc accgcccaga gactgaggcc agtctaccgg tttttaggtc ctctgttcca
     2521 ggagtttctt gctgccgtga ggttgactga actcctgagt tcagataggc aggaagacca
     2581 agatctggga ctttattatt tgagacaaat taactcacct ctaaaggcta tgagcatcta
     2641 ccacactttt ttgaaatatg tctccagcca cccttcatca aaggcagcgc caacagttgt
     2701 atctcatttg cttcagttgg tggatgagaa agagtcactg gagaacatgt ctgaaaatga
     2761 ggattacatg aagctccatc cagaagcttt gctgtggatt gagtgtctta gaggattgtg
     2821 gcagctgtct cctgaatctt tttctttgtt catttcagaa aacttattgc gcatttgtct
     2881 aaactttgct catgaaagca acacagttgc tgcatgttct ccagttattt tgcagttcct
     2941 tcgagggaga acactggatt tgaaagtttt gagtttacag tacttttggg accatccaga
     3001 aaccttgttg ttattgaaga gtattaagat ctcattaaat ggaaataact gggtacagag
     3061 aatcgatttt tcactcatag aaaaatcttt tgaaaaagta cagccaccaa ctatagatca
     3121 ggactatgcc attgcctttc aacctataaa tgaagtccag aaaaatttat ctgaaaagaa
     3181 acacataata aagaagtatg aggatatgaa acaccagatc ccactaaata ttagtactgg
     3241 ctactggaaa ctgtccccca agccatacaa gatccccaag ctggaagttc aagtgaccaa
     3301 cacaggtcca gcagatcaag cactgctcca ggtcctcatg gaagtcttct cagcttcaca
     3361 gagtattgag ttccgtttat ccgacagcag tggcttcctt gaaagcatcc gcccagctct
     3421 ggagctgagt aaggcctctg tcaccaagtg ttccatgtcc aggctggagc tcagcagaga
     3481 agatcagaag ctgcttctca ccctgcctac cctgcagtct ctcgaggtct cagagacaaa
     3541 ccagttacca gatcagctct tccataactt gcacaagttc ctgggcctga aagaactgtg
     3601 tgtgagacta gatagcaagc cggatgtgct ctcagtcctt cctggagagt tcccaaacct
     3661 ccatcacatg gagaagttat ccatcagaac ctccacggag tctgacctct ccaaactagt
     3721 taagttgatt cagaactctc caaatctcca tgttttccat ctgaagtgta atttcctttc
     3781 gaattgtgag cctctcatga ctgtgcttgc ttcctgcaag aaactcagag agattgagtt
     3841 ttctggacga tgctttgaag ccatgacctt tgtcaacatt ttgccaaatt ttgtttttct
     3901 gaagatattg aatcttagag accaacaatt cccagataaa gaaacatcag aaaagtttgc
     3961 ccaggctctg ggttctctca ggaacctgga gaaactgttc gttcccactg gggacgggat
     4021 tcaccaagtg gccaaactga ttgtccggca gtgtctgcag ctcccatgtc tccgagttct
     4081 tgtctttgca gagactctgg atgacgacag tgtactggaa attgccaagg gagcaaccag
     4141 gggaggcttc caaaaacttg agaacttaga ccttacactg aatcacaaga ttacagagga
     4201 aggatacaga aatttctttc aagtcctgga caacctgcca aacctgaaaa acctggacat
     4261 ttccaggcat atcccagaat gcattcaaat tcaggccatc actgtcaagg ctctgggtca
     4321 gtgtgtgtcc cgactgccca gcctcaccag gctcgggatg ctcagttggc tcctggatga
     4381 agaggacata aaagtgatta atgatgtgaa ggaaagacac cctcagtcca aacgcttgac
     4441 tgttcactgg aggtgggtag tgccattctc tcctgtcatt cagaagtgag cggccgcgtt
     4501 taaacgtcga cggtatcgat aagcttgata tcgaattccg cccccccccc taacgttact
     4561 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     4621 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     4681 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4741 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4801 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4861 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4921 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4981 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     5041 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5101 taatatggcc acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     5161 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtctg gcgagggcga
     5221 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     5281 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     5341 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     5401 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     5461 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5521 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5581 cgacaagcag aagaacggca tcaaggcgaa cttcaagatc cgccacaaca tcgaggacgg
     5641 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5701 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     5761 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5821 cgagctgtac aagtgataag aattcgataa aataaaagat tttatttagt ctccagaaaa
     5881 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     5941 gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga
     6001 gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg cccccggctc
     6061 agggccaaga acagatggtc cccgatgcgg tcccgccctc agcagtttct agagaaccat
     6121 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6181 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6241 ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg
     6301 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     6361 tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga
     6421 agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag
     6481 ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag
     6541 aagagctggg gagaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8701 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8761 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     8821 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8881 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     8941 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9001 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9061 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9121 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9181 cggcgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     9241 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     9301 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     9361 ggccacgatg cgtccggcgt agaggcgatt agtccaattt gttaaagaca ggatatcagt
     9421 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
     9481 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     9541 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa
     9601 ctgagaatag agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa
     9661 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
     9721 agatgcggtc ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
     9781 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     9841 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg
     9901 ccagtcctcc gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg
     9961 tttgcatccg aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg
    10021 cccacctcgg gggtctttca tttggaggtt ccaccgagat ttggagaccc ctgcccaggg
    10081 accaccgacc cccccgccgg gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt
    10141 ctttgtgcgt gtttgtgccg gcatctaatg tttgcgcctg cgtctgtact agttagctaa
    10201 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac ccggccgcaa
    10261 ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct gaggaaggga
    10321 gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg agaacctaaa
    10381 acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg
    10441 tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
    10501 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
    10561 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
    10621 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
    10681 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
    10741 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
    10801 ca
//
