LOCUS       sequence-96383-e       10659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mouse NAIP5 (B6 allele), expressed under the constitutive, 
            moderate-level retroviral LTR promoter for mammalian cell 
            expression; IRES-GFP reporter downstream of NAIP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Tenthorey JL, Kofoed EM, Daugherty MD, Malik HS, Vance RE
  TITLE     Molecular basis for specific recognition of bacterial ligands by 
            NAIP/NLRC4 inflammasomes.
  JOURNAL   Mol Cell. 2014 Apr 10;54(1):17-29. doi: 
            10.1016/j.molcel.2014.02.018. Epub 2014 Mar 20.
  PUBMED    24657167
REFERENCE   2  (bases 1 to 10659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.018"; journalName: "Mol Cell"; date: 
            "2014-04-10- 10"; volume: "54"; issue: "1"; pages: "17-29"
COMMENT     Sequence Label: mscv2.2-NAIP5
FEATURES             Location/Qualifiers
     source          1..10659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     57..79
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     95..111
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4394..4960
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4410..4962
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4554..4571)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4781..4800
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4974..4976,4977..4979,4980..5693)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5019..5040)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5280..5299)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5627..5648
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5746..6260
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6429..6445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6429..6445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6442..6464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6453..6469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6477..6483,6484..6501,6502..6507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6522..6543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6660..6677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6831..7419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6911..6930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7590..8381,8382..8450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8213..8232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8451..8555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8623..8641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8679..8701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8801..8820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9014..9036
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9164..9183
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             9383..9899
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    9963..10304
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             join(10371..10659,1..127)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
ORIGIN
        1 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgcccgt ctctccccct
       61 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
      121 aggcgccgga attagatcca ccatggctga gcatggggag tcctccgagg atcggatttc
      181 tgagattgat tatgaatttc tccccgagct ctctgctctt ctcggggtgg atgcatttca
      241 ggtggcaaag agccaagaag aagaagagca taaagaacgg atgaaaatga agaaaggttt
      301 taactcacag atgcgcagtg aagccaaacg actaaagacc tttgagacct atgacacgtt
      361 cagatcatgg acgccacagg agatggcagc tgctgggttt taccacacag gggtgagact
      421 tggcgttcag tgcttttgct gtagcttgat cctctttggt aacagcctca ggaagcttcc
      481 catagagaga cacaagaaat tacgtccgga atgtgagttc cttcagggca aagatgttgg
      541 taacattggc aagtatgaca tccgggtgaa gaggccagag aagatgctga gaggtggcaa
      601 agccaggtac catgaagagg aggccagact ggagtccttt gaggactggc cattttatgc
      661 ccatgggaca tcaccacgtg tactctcagc agctggcttt gtctttacag gtaaaaggga
      721 cactgtgcag tgtttctcct gtggcggaag cttgggcaac tgggaagaag gagatgaccc
      781 ctggaaggag catgccaagt ggttccccaa atgtgaattt cttcaaagta agaaatcctc
      841 agaggaaatt gcccagtata ttcaaagcta cgaaggattt gttcatgtaa cgggagaaca
      901 ttttgtgaag tcctgggtca gaagagaatt acctatggta tcagcttact gcaatgacag
      961 tgtcttcgct aatgaagaac taaggatgga catgtttaag gactggcccc aagaatcacc
     1021 tgtgggtgtt gaagctctag tcagagcagg ctttttctac actggcaaaa aggacattgt
     1081 ccggtgtttt tcctgtggag gatgtttgga aaagtgggca gaaggtgacg acccaatgga
     1141 agatcacatc aagttttttc ccgaatgtgt atttcttcaa accttgaagt cctctgcaga
     1201 agtaattcca acccttcaga gccaatatgc acttccagaa gccacggaaa ccacacgtga
     1261 aagcaaccat ggtgatgcag cagcagttca ttctacagtg gtggacttgg gtaggagtga
     1321 agcccagtgg tttcaagagg ccaggagtct gagtgagcag ctaagagaca actacactaa
     1381 agccactttc cgccacatga acttgccaga agtgtgctcc agccttggca ctgaccactt
     1441 gctcagctgc gatgtgtcca tcatttcaaa gcacatcagc cagcctgtgc aagaggccct
     1501 gacgatcccc gaggtcttct ccaatctcaa ctctgtcatg tgtgtggagg gggaaactgg
     1561 cagtggaaag acaaccttcc tgaagaggat agcttttctc tgggcatcag gatgctgccc
     1621 cctgttgtac aggttccagc tggtcttcta tctctccctt agttccatca caccagatca
     1681 gggactggcc aacatcatct gtgcccaact cctaggggca ggaggttgca ttagtgaagt
     1741 gtgtctgagc agcagcatcc agcagttaca acaccaggtg ctgttcctgt tggatgacta
     1801 cagtgggctg gcctcactcc cccaagccct acacacactg attacaaaaa actacttgtc
     1861 acggacctgc ttattgatcg ctgtgcatac aaacagggtc agagacatcc gcctatacct
     1921 aggtacaagt ctagagatcc aagagtttcc cttctataat actgtctctg tattacggaa
     1981 gtttttttca catgacataa tctgtgtgga aaagcttata atttacttta ttgataataa
     2041 agatttacag ggagtttaca agacccctct ctttgtagca gcagtatgta ctgactggat
     2101 tcaaaatgca tctgcccagg ataaatttca agatgtgaca cttttccagt cctacatgca
     2161 atacctgtcc ttaaaataca aagctacagc tgagcctctc caggccactg tgtcctcatg
     2221 tgggcagctg gccttgacag ggcttttctc atcatgcttt gagttcaata gtgatgacct
     2281 ggcagaggca ggagttgatg aagatgaaaa gcttaccacc ctcttgatga gcaaattcac
     2341 cgcccagaga ctgagaccag tctatcgatt tttaggtcct ctgttccagg agtttcttgc
     2401 tgccgtgagg ttgactgaac tcctgagttc agataggcag gaagaccaag atcttggact
     2461 ttattatttg agacaaatcg actcacctct gaaggcaata aactccttta acatattttt
     2521 gtattatgtc tccagccact cttcctcaaa ggcagcgcca acagttgtat ctcatttgct
     2581 tcagttggtg gatgagaaag agtcactgga gaacatgtct gaaaatgagg attacatgaa
     2641 gctccatcca caaacttttc tatggtttca gtttgttaga gggttgtggc tggtgtctcc
     2701 tgaatcttcc tcttcattcg tttcagaaca tttattgcgc cttgctctaa tctttgctta
     2761 tgaaagcaac acagttgctg aatgctctcc atttattttg caattccttc gaggaaaaac
     2821 actggcttta agagtactga atttacagta ctttagggac cacccagaaa gcctgttact
     2881 gttgaggagc ttaaaggttt ccataaatgg aaataaaatg tcatcttatg tagattattc
     2941 attcaagaca tattttgaaa acttacagcc accagctata gatgaggagt atacatctgc
     3001 ctttgagcat ataagtgaat ggaggagaaa ttttgctcaa gatgaggaga tcataaaaaa
     3061 ctatgaaaat atccgaccca gagccctacc agacatcagt gaagggtact ggaaactgtc
     3121 ccccaagcca tgcaagatcc ccaagctgga agttcaagtg aacaacacgg atgcagcaga
     3181 tcaagcactg ctccaggtcc tcatggaagt cttctcagct tcacagagta ttgagttccg
     3241 tttattcaac agcagtggct tccttgaaag catctgccca gctctggagc tgagtaaggc
     3301 ctctgtcacc aagtgttcca tgtccaggct ggagctcagc agagcagaac aggagctgct
     3361 tctcaccctg cctgccctgc agtctctcga ggtctcagag acaaaccagt taccagaaca
     3421 gctcttccat aacttgcaca agttcctggg cctgaaagaa ctgtgtgtga gactagatgg
     3481 caaaccgaat gtgctctcag tccttcctag agagttccca aacctccttc acatggagaa
     3541 gttatccatc caaacctcca cggagtctga cctctccaaa ctagttaaat tcatccagaa
     3601 ctttccaaat ctccatgttt tccatctgaa atgtgatttc ctttcaaatt gtgagtctct
     3661 catggctgtg cttgcttcct gcaagaaact cagagagatt gagttttctg gacgatgctt
     3721 tgaagccatg acctttgtca acattttgcc aaattttgtt tctctgaaga tactgaatct
     3781 taaagatcaa caatttccag ataaggaaac gtcagaaaag tttgcccagg ctctgggttc
     3841 tctcaggaac ctggaggaac tgctcgttcc cactggggac gggattcacc aagtggccaa
     3901 actgattgtc cggcagtgtc tgcagcttcc gtgcctccga gttctcacct ttcacgacat
     3961 cttggacgat gacagtgtga ttgaaattgc cagggcggca accagtggag gtttccagaa
     4021 acttgagaac ttagatattt ccatgaatca caagattacc gaggaaggat atagaaattt
     4081 ctttcaagcc ctggacaact tgccaaacct acaagagctg aacatctgca ggaatatccc
     4141 aggacgcatt caagttcagg ccaccactgt caaggctctg ggtcaatgtg tgtcccgact
     4201 gcccagcctc atcagactgc acatgctcag ttggctcctg gatgaagagg acatgaaagt
     4261 gattaatgat gtgaaggaaa gacatcccca gtctaaacgc ttgattatct tctggaaatt
     4321 gatagtccca ttctctcctg ttatcctgga gtgagtttaa acgtcgacgg tatcgataag
     4381 cttgatatcg aattccgccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     4441 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     4501 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     4561 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     4621 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     4681 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     4741 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     4801 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     4861 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     4921 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
     4981 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5041 taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc tacggcaagc
     5101 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5161 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     5221 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5281 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5341 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     5401 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     5461 aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     5521 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     5581 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     5641 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tgataagaat
     5701 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     5761 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac
     5821 tgagaataga gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa
     5881 caggatatct gtggtaagca gttcctgccc ccggctcagg gccaagaaca gatggtcccc
     5941 gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     6001 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     6061 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     6121 cagtcctccg atagactgcg tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     6181 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     6241 cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt tggagaacaa cattctgagg
     6301 gtaggagtcg aatattaagt aatcctgact caattagcca ctgttttgaa tccacatact
     6361 ccaatactcc tgaaatagtt cattatggac agcgcagaag agctggggag aattaattcg
     6421 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6481 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6541 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6601 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6661 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6721 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6781 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6841 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6901 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6961 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7021 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7081 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7141 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7201 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7261 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7321 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7381 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7441 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7501 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7561 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7621 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7681 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7741 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7801 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7861 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7921 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7981 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8041 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8101 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8161 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8221 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8281 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8341 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8401 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8461 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8521 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8581 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8641 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8701 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     8761 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     8821 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     8881 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8941 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     9001 acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca
     9061 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     9121 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     9181 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     9241 ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc
     9301 aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa aagattttat
     9361 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     9421 aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc
     9481 aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt
     9541 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
     9601 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     9661 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     9721 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
     9781 gcccgggtac ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggactcg
     9841 ctgatccttg ggagggtctc ctcagattga ttgactgccc acctcggggg tctttcattt
     9901 ggaggttcca ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag
     9961 gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt tgtgccggca
    10021 tctaatgttt gcgcctgcgt ctgtactagt tagctaacta gctctgtatc tggcggaccc
    10081 gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt cccagggact
    10141 ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat ccgaccccgt
    10201 caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
    10261 ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc tgcagcatcg
    10321 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg
    10381 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
    10441 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
    10501 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
    10561 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
    10621 cctgggaagc cttggctttt gacccccctc cctgggtca
//
