LOCUS       sequence-96388-e        4959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged catalytic mutant of TUT4 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Liu X, Zheng Q, Vrettos N, Maragkakis M, Alexiou P, Gregory BD, 
            Mourelatos Z
  TITLE     A MicroRNA Precursor Surveillance System in Quality Control of 
            MicroRNA Synthesis.
  JOURNAL   Mol Cell. 2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017.
  PUBMED    25175028
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2014 Aug 27. pii: S1097-2765(14)00610-8. doi: 
            10.1016/j.molcel.2014.07.017."
COMMENT     Sequence Label: pCI-FLAG-mTUT4(D1028A)
FEATURES             Location/Qualifiers
     source          1..4959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgacaaggaa gagcccaaga cttctaaaaa cgaaaatcat
       61 gaaccaaaga agaatattat ttgtgaagaa agcaaagcag ttaaaattat atctaatcaa
      121 accttgaagc ctagaaatga taagtcagaa attgggacca gctctctgaa tagaaatagt
      181 agtaaaaaaa ccaagcaaaa tgatatttgt atagaaaaaa cagaagctaa atcatgtaaa
      241 gtaaacgctg ccagtgttcc aggccctaaa gatttggggt tagtacatcg agatcaaagt
      301 cattgcaaaa tgaaaaagtt accaaattca cccatgaaag cccagaaggg atcttcacag
      361 acaaaattag aaaagacacc tagtttacag acaaaagcag aaaaagtacc aaagtctcca
      421 aatttaccag taaaagcaga aaaagcgccc tgtacaacag cagaagcaac aacagaaaaa
      481 gcacttaatt cacagagaaa agaagaaaac acacccactt ctcagatgaa attacagaag
      541 acacccagat cacctttaga gccagaaaat gtgcccagtt tactattgaa agaaaatgtg
      601 aaacagacag aatcacaaca gactgggaag aaacttacaa gctcctttgt ttctatggat
      661 aaaaggaaga gtgaagctct acagggagaa aagtcagctc tggaaaactc atcactatct
      721 cagaagcagc agactcagac agataatatt gctgattctg atgacagtgc ttcagggatt
      781 gaagatacag cagatgattt gagtaaaatg aagagtgagg aatctaacaa agaaaattct
      841 tctgagatgg actatttaga aaatgccact gtaatagatg aatctgcttt gacacctgag
      901 cagaggctag gcttgaaaca agcagaagaa cgtctagaaa gagatcacat cttccgactt
      961 gaaaaacggt caccagaata taccaattgc cgctatctat gcaaactttg cttaattcac
     1021 attgaaaaca tccaaggagc acataaacat ataaaagaaa aacgacataa gaaaaatatt
     1081 ttggaaaaac aagaagagag tgaacttcgg tctcttccat ctccttcctc tgctcacttg
     1141 gctgctttaa gtgttgcagt tgttgagtta gcaaaagaac aaggaataac agatgatgac
     1201 ctcagaatcc gccaggacat tgtggaggaa atgtcaaagg ttataatgac atttttacca
     1261 gaatgttcac tgaggttgta tggttcatct ctgacaaagt ttgctctgaa aagcagtgat
     1321 gttaatatag atataaaatt tcctcccaag atgaatcatc cagatcttct gatacaagtg
     1381 cttggaattc tgaaaaaaag tgcattatat atagatgtgg aatctgattt tcatgctaaa
     1441 gttcctgttg tggtgtgcaa agatcggaaa agtgctttac tgtgtagagt aagtgcagga
     1501 aatgatatgg cgtgtctcac aactgactta cttgctgctc ttggcaaagt ggaacccgtc
     1561 ttcactccct tagtgctagc ttttcgctat tgggctaagc tgtgctatat tgactcccaa
     1621 actgatggtg gaatcccttc ttactgcttt gctttgatgg tgatgttttt tcttcaacaa
     1681 agaaaacccc ctcttcttcc ttgcttactt ggaagttgga ttgaaggctt tgacccgaaa
     1741 agaatggatg actttcagct gaagggcata gtagaagaga agtttgtgaa gtgggaatat
     1801 aattcaagta gtgcaactga gaaaaactta attgctgatg aaaacaaagc taaggcagac
     1861 gaaccaaaag atgataccaa gaagacagaa acagacaacc aaagtaatgc tgcgaaggca
     1921 aaacatggca aatctccttt aacattggaa gcaccaaatc aggtaccctt gggacagttg
     1981 tggttggagc tgctaaaatt ttacacactg gattttgctt tggaggaata tgtcatatgt
     2041 gtacggatac aagatattct aactagagag aacaaaaact ggcctaaaag gcggatagcc
     2101 attgaagatc cattttcagt caagaggaat gttgcccgga gcttaaacag ccagcttgta
     2161 tacgaatatg ttgtggaaag attcagagca gcttatcgat attttgcctg tcctcaaaag
     2221 aagggtggaa ataaatctac aatggatcct aagaagaaag agaaggggaa gctaagcagt
     2281 aagaaaccag tgaagtctga ttgttcggca accaattgtt gcattcttgg ggaaagtgca
     2341 gaaaaaatac atatggaaag aggccaacct gctaaacatg atgaaacgga gtttacatca
     2401 cagagatgta ttgttgataa tgacagttta ttggtaaatg aactaggttt ggctaaccat
     2461 ggacaagctt cttcatctct ttctactgcc agtggaggca gtgacttaaa gcaaaaatca
     2521 gctgagaaac aaggtgattt aacaccttca gaaacttcct taaagaagga actcagccag
     2581 tgtatttgca ttgggacccc tgatggagct gaatctgctg gaacagactg cagatcaaac
     2641 ttagaaatgg acagttcaca tcagattgtg tgcaacaatg tatctgctac ctcttgcaac
     2701 tgcaaagcta cagaagttac ttctgacctt gttgatgagg ataacctccc ctcccaggaa
     2761 ttatattatg tgtttgataa gttcatttta acctctggca agccgccaac aatagtatgc
     2821 agcatctgca aaaaggatgg ccattcaaaa aatgattgtc cagaggattt caggaaaatt
     2881 gatctaaaac ctctaccacc aatgacaaac cgatttcgag aaatacttga tttagtatgt
     2941 aaaagatgtt ttgatgaatt atcaccgcct tgctctgaac aacacaacag ggaacaaatt
     3001 ttaattggct tggaaaagtt tattcaaaaa gaatatgatg aaaaagcaag attgtgctta
     3061 tttggttctt ctaagaatgg atttggattc cgagatagtg atctggctat ttgtatgact
     3121 ctggaaggcc atgaaaatgc agagaaatta aactgtaaag aaataattga aaatttggcg
     3181 aaaattctta agaggcatcc aggtttaaga aacattctgc ctataactac tgccaaagtg
     3241 cctattgtaa aatttgaaca caggagaagt ggactagaag gtgatatcag tttatataac
     3301 acattggctc aacacaacac aagaatgcta gctacttatg cagctattga tcctagagta
     3361 cagtacttgg gatatactat gaaagtgttt gcaaagcgct gtgacattgg agatgcatct
     3421 cggggaagtt tatcttcata tgcttacatc cttatggtgc tgtactttct gcagcaaaga
     3481 aagccaccag ttatcccagt tctacaagag atctttgatg ggaagcagat tccgcagaga
     3541 atggttgatg gatggaatgc ctttttcttt gataaaacag aagagctgaa aaagcgttta
     3601 ccttcacttg ggaagaacac ggaatcactg ggagagcttt ggttaggact gcttcgcttc
     3661 tatacagaag agtttgattt taaggagtat gtaatcagta ttcggcagaa gaagctgctg
     3721 accacttttg aaaagcagtg gacttctaaa tgcattgcaa ttgaagatcc ttttgacttg
     3781 aatcataacc ttggtgctgg tgtttctaga aaaatgacta attttattat gaaggcgttt
     3841 atcaatggaa ggaaactttt tggtaccccc ttttatccac tcattggcag agaagctgag
     3901 tacttctttg attcaagagt actaacagat ggagagttgg ctcctaatga tcgttgttgc
     3961 cgtgtgtgtg gaaaaatagg tcactacatg aaagactgtc ccaaaaggaa aagactaaag
     4021 aagaaagaca gtgaagaaga gaaggaaggg aacgaagaag aaaaagactc ccgtgacctt
     4081 cttgactccc gagaccttag gtgtttcata tgtggagatg ctggacatgt tcgccgcgaa
     4141 tgcccagagg tcaagatggc ccgccagagg aatagtagtg tggcagcagc ccagctggtc
     4201 cgtaaccttg taaatgctca gcaggtggct ggttcagcgc agcaacagag tgaccagtcc
     4261 ataaggacca gacagtcttc agaatgttct gattcaccat cttattctcc tcagcctcag
     4321 ccttttccac agaattctcc ccaaccatct gctcttcccc cacctccatc ccagccagga
     4381 tcccagccta agcttggccc accccagcag ggagggcagc cccctcatca ggttcagatg
     4441 cccttgtata actttcctca gtcacctcca gctcattatt cacccatgca cagtatgggg
     4501 ttactgccaa tgcaccccct gcagatccct gccccatcgt ggcccatcca cggcccgatg
     4561 ctgcactctg cacctggcag cacccccagc aacattggct tgaatgatcc cagcatcatc
     4621 tttgcacagc ctgctgccag gcccatggca attcccagcc cttctcacga tggacactgg
     4681 cctcggaccg tggctcccaa ttccctggtg aacaatggtg cagtgggtaa ttcggagcca
     4741 cgctttcgag gactaaatcc tccaattcca tgggaacatg caccacgcca tttccccctt
     4801 gtcccagctt cgtggcctta cggtttgcat caaaatttca tgcatcaggg aaatccaaga
     4861 tttcagccca aacccttcta tgctcaagca gacagatgtg ccacgcgtcg ctgtagagag
     4921 cgctgtcccc acccaccacg aggaaacgtg tccgagtaa
//
