LOCUS       sequence-96406-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK295
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(281..299)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        367..471
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(690..709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacgagctgt acaagtaact cgaggaagcg cagtacataa tgctgcgcag tgttgccaca
       61 taaccactat attaaccatt tatctagcgg acgccaaaaa ctcaatgtat ttctgaggaa
      121 gcgtggtgca taatgccacg cagcgtctgc ataactttta ttatttcttt tattaatcaa
      181 caaaattttg tttttaacat ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
      241 gggaattcga gctcgggaat taattcttga agacgaaagg gcctcgtgat acgcctattt
      301 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
      721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt gg
//
