LOCUS       sequence-9641-ex        3001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Lipson KL, Ghosh R, Urano F
  TITLE     The role of IRE1alpha in the degradation of insulin mRNA in 
            pancreatic beta-cells.
  JOURNAL   PLoS ONE. 2008  . 3(2):e1648.
  PUBMED    18286202
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(2):e1648."
COMMENT     Sequence Label: hIRE1a K599A
FEATURES             Location/Qualifiers
     source          1..3001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..110
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2948..2972)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagctcgggc ccaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
       61 tgataagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac
      121 caattcagtc gactggatcc ggtaccgaat tcgccatgcc ggcccggcgg ctgctgctgc
      181 tgctgacgct gctgctgccc ggcctcggga tttttggaag taccagcaca gtgacgcttc
      241 ctgaaacctt gttgtttgtg tcaacgctgg atggaagttt gcatgctgtc agcaagagga
      301 caggctcaat caaatggact ttaaaagaag atccagtcct gcaggtccca acacatgtgg
      361 aagagcctgc ctttctccca gatcctaatg atggcagcct gtatacgctt ggaagcaaga
      421 ataatgaagg cctgacgaaa cttcctttta ccatcccaga attggtgcag gcatccccat
      481 gccgaagttc agatggaatc ctctacatgg gtaaaaagca ggacatctgg tatgttattg
      541 acctcctgac cggagagaag cagcagactt tgtcatcggc ctttgcagat agtctctgcc
      601 catcaacctc tcttctgtat cttgggcgaa cagaatacac catcaccatg tacgacacca
      661 aaacccgaga gctccggtgg aatgccacct actttgacta tgcggcctca ctgcctgagg
      721 acgacgtgga ctacaagatg tcccactttg tgtccaatgg tgatgggctg gtggtgactg
      781 tggacagtga atctggagac gtcctgtgga tccaaaacta cgcctcccct gtggtggcct
      841 tttatgtctg gcagcgggag ggtctgagga aggtgatgca catcactgtc gctgtggaga
      901 ccctgcgcta tctgaccttc atgtctgggg aggtggggcg catcacaaag tggaagtacc
      961 cgttccccaa ggagacagag gccaagagca agctgacgcc cactctgtat gttgggaaat
     1021 actctaccag cctctatgcc tctccctcaa tggtacacga gggggttgct gtcgtgcccc
     1081 gcggcagcac acttcctttg ctggaagggc cccagactga tggcgtcacc atcggggaca
     1141 agggggagtg tgtgatcacg cccagcacgg acgtcaagtt tgatcccgga ctcaaaagca
     1201 agaacaagct caactacttg aggaattact ggcttctgat aggacaccac gtgtctcggg
     1261 atgtggagga gaagcccgcc catgcccctg cccggcccga ggcccccgtg gactccatgc
     1321 ttaaggacat ggctaccatc atcctgagca ccttcctgct gattggctgg gtggccttca
     1381 tcatcaccta tcccctgagc atgcatcagc agcagcagct ccagcaccag cagttccaga
     1441 aggaactgga gaagatccag ctcctgcagc agcagcagca gcagctgccc ttccacccac
     1501 ctggagacac ggctcaggac ggcgagctcc tggacacgtc tggcccgtac tcagagagct
     1561 cgggcaccag cagccccagc acgtccccca gggcctccaa ccactcgctc tgctccggca
     1621 gctctgcctc caaggctggc agcagcccct ccctggaaca agacgatgga gatgaggaaa
     1681 ccagcgtggt gatagttggg aaaatttcct tctgtcccaa ggatgtcctg ggccatggag
     1741 ctgagggcac aattgtgtac cggggcatgt ttgacaaccg cgacgtggcc gtggcgagga
     1801 tcctccccga gtgttttagc ttcgcagacc gtgaggtcca gctgttgcga gaatcggatg
     1861 agcacccgaa cgtgatccgc tacttctgca cggagaagga ccggcaattc cagtacattg
     1921 ccatcgagct gtgtgcagcc accctgcaag agtatgtgga gcagaaggac tttgcgcatc
     1981 tcggcctgga gcccatcacc ttgctgcagc agaccacctc gggcctggcc cacctccact
     2041 ccctcaacat cgttcacaga gacctaaagc cacacaacat cctcatatcc atgcccaatg
     2101 cacacggcaa gatcaaggcc atgatctccg actttggcct ctgcaagaag ctggcagtgg
     2161 gcagacacag tttcagccgc cgatctgggg tgcctggcac agaaggctgg atcgctccag
     2221 agatgctgag cgaagactgt aaggagaacc ctacctacac ggtggacatc ttttctgcag
     2281 gctgcgtctt ttactacgta atctctgagg gcagccaccc ttttggcaag tccctgcagc
     2341 ggcaggccaa catcctcctg ggtgcctgca gccttgactg cttgcaccca gagaagcacg
     2401 aagacgtcat tgcacgtgaa ttgatagaga agatgattgc gatggatcct cagaaacgcc
     2461 cctcagcgaa gcacgtgctc aaacacccgt tcttctggag cctagagaag cagctccagt
     2521 tcttccagga cgtgagcgac agaatagaaa aggaatccct ggatggcccg atcgtgaagc
     2581 agttagagag aggcgggaga gccgtggtga agatggactg gcgggagaac atcactgtcc
     2641 ccctccagac agacctgcgt aaattcagga cctataaagg tggttctgtc agagatctcc
     2701 tccgagccat gagaaataag aagcaccact accgggagct gcctgcagag gtgcgggaga
     2761 cgctggggtc cctccccgac gacttcgtgt gctacttcac gtctcgcttc ccccacctcc
     2821 tcgcacacac ctaccgggcc atggagctgt gcagccacga gagactcttc cagccctact
     2881 acttccacga gcccccagag ccccagcccc cagtgactcc agacgccctc tgactcgaga
     2941 tatctagacc cagctttctt gtacaaagtt ggcattataa gaaagcatgc ttatcaattg
     3001 t
//
