LOCUS       sequence-96418-e         437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 nuclease and gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T
  TITLE     Multiplex genome engineering in human cells using all-in-one 
            CRISPR/Cas9 vector system.
  JOURNAL   Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400.
  PUBMED    24954249
REFERENCE   2  (bases 1 to 437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-23"; volume: "4:5400. doi"; pages: " 
            10.1038/srep05400"
COMMENT     Sequence Label: pX330A-1x6
FEATURES             Location/Qualifiers
     source          1..437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(304..324)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(331..396)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      396..405
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccaccttgg ccatctcgtt gctgaagatc tcttgcagat agcagatccg ggttcttccg
       61 tctggtgtat cttcttctgg cggttctctt cagccgggtg gcctcggctg tttcgccgct
      121 gtcgaacagc agggctccga tcaggttctt cttgatgctg tgccggtcgg tgttgcccag
      181 caccttgaat ttcttgctgg gcaccttgta ctcgtcggtg atcacggccc agcccacaga
      241 gttggtgccg atgtccaggc cgatgctgta cttcttgtcg gctgctggga ctccgtggat
      301 accgaccttc cgcttcttct ttggggccat cttatcgtca tcgtctttgt aatcaatatc
      361 atgatccttg tagtctccgt cgtggtcctt atagtccatg gtggcaccgg tccaacctga
      421 aaaaaagtga tttcagg
//
