LOCUS       sequence-96431-e         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a granzyme B FRET reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Choi PJ, Mitchison TJ
  TITLE     Imaging burst kinetics and spatial coordination during serial 
            killing by single natural killer cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6488-93. doi: 
            10.1073/pnas.1221312110. Epub 2013 Apr 1.
  PUBMED    23576740
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1221312110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-04-16- 16"; volume: "110"; issue: "16"; pages: "6488-93"
COMMENT     Sequence Label: pLNCX2-CFP-VGPDFGR-YFP
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..303)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(94..115)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     443..462
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     702..723
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attttatcga tgttaggcca ttaaggcctg tcgagaggcc ttgaattctt acttgtacag
       61 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      121 gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg
      181 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      241 gccgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt
      301 gtcggcggtg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      361 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      421 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      481 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      541 ggggtagcgg gcgaagcact gcaggccgta gcccagggtg gtcacgaggg tgggccaggg
      601 cacgggcagc ttgccggtgg tgcagatcag cttcagggtc agcttgccgt aggtggcatc
      661 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      721 caggatgggc accaccccgg tgaacagctc ctcgcc
//
