LOCUS       sequence-96434-e         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Guo L, Giasson BI, Glavis-Bloom A, Brewer MD, Shorter J, Gitler AD, 
            Yang X
  TITLE     A Cellular System that Degrades Misfolded Proteins and Protects 
            against Neurodegeneration.
  JOURNAL   Mol Cell. 2014 Jul 3;55(1):15-30. doi: 10.1016/j.molcel.2014.04.030.
            Epub 2014 May 29.
  PUBMED    24882209
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.04.030"; journalName: "Mol Cell"; date: 
            "2014-07-3- 3"; volume: "55"; issue: "1"; pages: "15-30"
COMMENT     Sequence Label: Flag-PML IV/pRK5
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(47..70)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agttgggcca tggcggccaa gcttctgcag gtcgagcggc cgcctacttg tcatcgtcgt
       61 ccttgtagtc gagtcgtcga cctaaattag aaaggggtgg gggtagcccc aggagaaccc
      121 actttcattg tcaatcttga ggtcaaagaa aaccagaggt ctgtcttctg cttgggggtc
      181 agcaaggttc tcgtccagga ccctgagggt gctggggcct tccagctgga ggtcactgga
      241 ctcactgctg ctgtcatcca gctctcggga ggacgagttt tcggcatctg agtcttccga
      301 gctgctgatc accacaacgc gttcctctgc ctccccggcg ccactggcca cgtggttgct
      361 gttgggcagg aagacctcac ttcctatgac ggggctcctg gggctaggcg gtccatccag
      421 gtggggtggt gagactgcct tggaggtgct gggcctgggc tgctccgggg agctccgagc
      481 caaccttgcc tccttcccct cctcagactc catcttgatg accttcctgg ggcactgggt
      541 ctggctgcac ttcctcttct gggctgtcgt tgtattggag acatcctctc cataggaagg
      601 gcctttgatg gagaaggcgt acactggcac ggggtgtgcc ccgggcactg actgtaccac
      661 agccataggc tgggcttcag ccagccccag ggcctgaacc tgggccttca ctctctctgc
      721 ctcctcgggc aggtcaacgt caatagggtc cctgggagtg ctggcagcct ctgggctggc
      781 tttcttggat acagctgcat ctttcccctg g
//
