LOCUS       sequence-96435-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express yellow fluorescent protein (YFP) with strong T7 RBS at a low
            gene dosage (3-4 copies/cell).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Subramaniam AR, Pan T, Cluzel P
  TITLE     Environmental perturbations lift the degeneracy of the genetic code 
            to regulate protein levels in bacteria.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Feb 5;110(6):2419-24. doi: 
            10.1073/pnas.1211077110. Epub 2012 Dec 31.
  PUBMED    23277573
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1211077110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-02-5- 5"; volume: "110"; issue: "6"; pages: "2419-24"
COMMENT     Sequence Label: pZS*11-yfp0
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(19..37,38..43,44..60,61..66,67..92)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    44..62
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tttcgtcttc acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga
       61 gatactgagc acatcagcag gacgcactga ccgaattcaa taattttgtt taactttaag
      121 aaggagatgg taccatgagc aaaggtgaag aactgttcac cggcgttgtg ccaattctgg
      181 ttgagctgga tggtgacgtg aatggccaca aattttccgt gtctggtgaa ggcgagggtg
      241 atgctactta tggcaaactg actctgaaac tgatctgtac caccggcaaa ctgcctgttc
      301 catggccaac tctggtcact actctgggtt acggcctgat gtgttttgcg cgttacccgg
      361 atcacatgaa acagcatgac ttcttcaaat ctgccatgcc ggaaggctat gtccaagaac
      421 gtacgatctt tttcaaggac gacggcaact ataaaacccg tgccgaagtt aaattcgagg
      481 gtgacaccct ggttaaccgc atcgaactga aaggcattga cttcaaagag gacggcaaca
      541 ttctgggtca caagctggaa tacaactaca actcccacaa cgtttacatt actgctgaca
      601 agcagaaaaa
//
