LOCUS       sequence-96437-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNF4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Guo L, Giasson BI, Glavis-Bloom A, Brewer MD, Shorter J, Gitler AD, 
            Yang X
  TITLE     A Cellular System that Degrades Misfolded Proteins and Protects 
            against Neurodegeneration.
  JOURNAL   Mol Cell. 2014 Jul 3;55(1):15-30. doi: 10.1016/j.molcel.2014.04.030.
            Epub 2014 May 29.
  PUBMED    24882209
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.04.030"; journalName: "Mol Cell"; date: 
            "2014-07-3- 3"; volume: "55"; issue: "1"; pages: "15-30"
COMMENT     Sequence Label: HA-RNF4/pRK5
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(28..51)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(640..666)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(640..666)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(722..741)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(754..772)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(755..772)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agttgggcca tggcggccaa gctttcactt gtcatcgtcg tccttgtagt cgagtcagtc
       61 gactatataa atggggtggt accgtttgtg gttgatcttt ttcctacaag ttgggcaagt
      121 attagcattc ttcagggaat cacggaggca ctggctacag aagacatggc cgcattctgt
      181 ggaaacgatg agacgtccat tctgcacgat ctctgagtat ccgtccatgc agatgggaca
      241 actgacagta cctgagggcc tgaggcctgt agcgccctca tccctggcgt ttctgggagt
      301 atgggtagtc acatatacgt ctctgtccct ggacaactcc tcatcgtcac tgctcaccac
      361 acagctgtca gcatggtcct ggggcagcct cctagcattc ctccttggtc ttcttctttc
      421 gtcaacaatc acaacagagt cattgtgagt cagatcaacc accacaggct ctaaagattc
      481 acaagtgagg tccacaattt catctccagc agtttccacg agttctatgg gttctgcttc
      541 caaggagatc tcgggggtgg aggttgcttc ccgagttcgc ttctgagctt gtctagaatt
      601 tattgctcca ccacgacgct ttcttgtact catggatctg gcgtagtcgg gcacgtcgta
      661 ggggtagccc atggcgaatt caatcgatag aaccgaggtg cagttggacc tgggagtgga
      721 cacctgtgga gagaaaggca aagtggatgt tattctatag tgtcacctaa atcgtatgtg
      781 tatgatacat aaggttatgt attaattgta gccgcgttct aacgaagcca aggg
//
