LOCUS       sequence-96438-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNF4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Guo L, Giasson BI, Glavis-Bloom A, Brewer MD, Shorter J, Gitler AD, 
            Yang X
  TITLE     A Cellular System that Degrades Misfolded Proteins and Protects 
            against Neurodegeneration.
  JOURNAL   Mol Cell. 2014 Jul 3;55(1):15-30. doi: 10.1016/j.molcel.2014.04.030.
            Epub 2014 May 29.
  PUBMED    24882209
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.04.030"; journalName: "Mol Cell"; date: 
            "2014-07-3- 3"; volume: "55"; issue: "1"; pages: "15-30"
COMMENT     Sequence Label: HA-RNF4/pRK5
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..81
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             55..81
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             670..693
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      704..713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    717..851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(754..773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     808..827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcgccat gggctacccc
       61 tacgacgtgc ccgactacgc cagatccatg agtacaagaa agcgtcgtgg tggagcaata
      121 aattctagac aagctcagaa gcgaactcgg gaagcaacct ccacccccga gatctccttg
      181 gaagcagaac ccatagaact cgtggaaact gctggagatg aaattgtgga cctcacttgt
      241 gaatctttag agcctgtggt ggttgatctg actcacaatg actctgttgt gattgttgac
      301 gaaagaagaa gaccaaggag gaatgctagg aggctgcccc aggaccatgc tgacagctgt
      361 gtggtgagca gtgacgatga ggagttgtcc agggacagag acgtatatgt gactacccat
      421 actcccagaa acgccaggga tgagggcgct acaggcctca ggccctcagg tactgtcagt
      481 tgtcccatct gcatggacgg atactcagag atcgtgcaga atggacgtct catcgtttcc
      541 acagaatgcg gccatgtctt ctgtagccag tgcctccgtg attccctgaa gaatgctaat
      601 acttgcccaa cttgtaggaa aaagatcaac cacaaacggt accaccccat ttatatagtc
      661 gactgactcg actacaagga cgacgatgac aagtgaaagc ttggccgcca tggcccaact
      721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      841 atgtctggat cgg
//
