LOCUS       sequence-96441-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of myc p31Comet in the genome and 
            Tet-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Westhorpe FG, Tighe A, Lara-Gonzalez P, Taylor SS
  TITLE     p31comet-mediated extraction of Mad2 from the MCC promotes efficient
            mitotic exit.
  JOURNAL   J Cell Sci. 2011 Nov 15;124(Pt 22):3905-16. doi: 10.1242/jcs.093286.
            Epub 2011 Nov 18.
  PUBMED    22100920
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2011-11-15"; volume: "124(Pt 22):3905-16. doi"; pages:
            " 10.1242/jcs.093286. Epub 2011 Nov 18"
COMMENT     Sequence Label: pcDNA5 FRT TO myc p31Comet
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..31
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             326..355
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      121 ccagcctccg gactctagcg tttaaactta agcttggtac cccggggaat tcggcagatc
      181 ccgaggtccg acagcgcccg gcccagatcc ccacgcctgc caggagcaag ccgagagcca
      241 gccggccggc gcactccgac tccgagcagt ctctgtcctt cgacccgagc cccgcgccct
      301 ttccgggacc cctgccccgc ggatggagca gaagctcatc tcagaagaag acctcgaggg
      361 atccgcggcg ccggaggcgg aggttctgtc ctcagccgca gtccctgatt tggagtggta
      421 tgagaagtcc gaagaaactc acgcctccca gatagaacta cttgagacaa gctctacgca
      481 ggaacctctc aacgcttcgg aggccttttg cccaagagac tgcatggtac cagtggtgtt
      541 tcctgggcct gtgagccagg aaggctgctg tcagtttact tgtgaacttc taaagcatat
      601 catgtatcaa cgccagcagc tccctctgcc ctatgaacag cttaagcact tttaccgaaa
      661 accttctccc caggcagagg agatgctgaa gaagaaacct cgggccacca ctgaggtgag
      721 cagcaggaaa tgccaacaag ccctggcaga actggagagt gtcctcagcc acctggagga
      781 cttctttgca cggacactag taccgcgagt gctgattctc ctt
//
