LOCUS       sequence-96442-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI 
            mutated)-P2A-EBFP2-4xFF5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK332
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(401..419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        487..591
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgagctgt acaagtaaac tagtaaaagc actctgattt gacaattaaa gcactctgat
       61 ttgacaatta aagcactctg atttgacaat taaagcactc tgatttgaca attaaagcgg
      121 ccgcgactct agatcatact cgaggaagcg cagtacataa tgctgcgcag tgttgccaca
      181 taaccactat attaaccatt tatctagcgg acgccaaaaa ctcaatgtat ttctgaggaa
      241 gcgtggtgca taatgccacg cagcgtctgc ataactttta ttatttcttt tattaatcaa
      301 caaaattttg tttttaacat ttcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
      361 gggaattcga gctcgggaat taattcttga agacgaaagg gcctcgtgat acgcctattt
      421 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      661 cacccagaaa cgctggtgaa agtaaaagat gctgaa
//
