LOCUS       sequence-96445-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of Myc ArA in the genome and Tet-inducible 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Girdler F, Gascoigne KE, Eyers PA, Hartmuth S, Crafter C, Foote KM, 
            Keen NJ, Taylor SS
  TITLE     Validating Aurora B as an anti-cancer drug target.
  JOURNAL   J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.
  PUBMED    16912073
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-09-1"; volume: "119(Pt 17)"; pages: "3664-75. 
            Epub 2006 Aug 15"
COMMENT     Sequence Label: pcDNA5 FRT TO Myc ArA WT
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             331..360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccccgg ggaattcggc
      181 agatcccgag gtccgacagc gcccggccca gatccccacg cctgccagga gcaagccgag
      241 agccagccgg ccggcgcact ccgactccga gcagtctctg tccttcgacc cgagccccgc
      301 gccctttccg ggacccctgc cccgcggatg gagcagaagc tcatctcaga agaagacctc
      361 gagggatccg accgatctaa agaaaactgc atttcaggac ctgttaaggc tacagctcca
      421 gttggaggtc caaaacgtgt tctcgtgact cagcaatttc cttgtcagaa tccattacct
      481 gtaaatagtg gccaggctca gcgggtcttg tgtccttcaa attcttccca gcgcgttcct
      541 ttgcaagcac aaaagcttgt ctccagtcac aagccggttc agaatcagaa gcagaagcaa
      601 ttgcaggcaa ccagtgtacc tcatcctgtc tccaggccac tgaataacac ccaaaagagc
      661 aagcagcccc tgccatcggc acctgaaaat aatcctgagg aggaactggc atcaaaacag
      721 aaaaatgaag aatcaaaaaa gaggcagtgg gctttggaag actttgaaat tggtcgccct
      781 ct
//
