LOCUS       sequence-96446-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH
  TITLE     Crystal structure of NusA from Thermotoga maritima and functional 
            implication of the N-terminal domain.
  JOURNAL   Biochemistry. 2003 Nov 25. 42(46):13429-37.
  PUBMED    14621988
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2003 Nov 25. 42(46):13429-37."
COMMENT     Sequence Label: pSKB3.TM1777
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..93
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cgattacgat
       61 atcccaacga ccgaaaacct ttacttccag ggccatatga acataggctt gctggaagcc
      121 ctcgaccagc tggaggaaga aaaaggaatt tccaaagaag aggtcattcc tatccttgaa
      181 aaagcactgg tgagcgctta caggaagaac tttggaaatt ccaagaacgt ggaggttgtt
      241 atagatagga acacgggaaa cataaaagtg tatcagctcc tcgaagttgt ggaagaagtg
      301 gaagatccag caacacagat atctctcgag gaggcgaaaa agatcgaccc cctcgcggaa
      361 gttgggtcta ttgtgaagaa ggaactgaac gttaagaatt ttggaagaat agccgcgcag
      421 acggcgaagc aggttctcat tcagagaatc agagaactcg agaaggagaa acagttcgag
      481 aagtattccg aactcaaagg aacggttaca accgctgaag tcataagagt catgggcgag
      541 tgggcagaca tcagaatagg aaagctcgag acaaggcttc caaagaaaga gtggatcccc
      601 ggtgaggaaa tcaaagccgg tgatctggtg aaggtctaca tcatcgatgt ggttaaaaca
      661 accaaggggc cgaagatact cgtgagcagg agagtaccgg agttcgtaat tggcctgatg
      721 aaactcgaaa ttccggaagt ggagaatgga atcgtggaaa taaaggctat cgccagagaa
      781 cccggtgttc gaacaaaggt ggcagttgca tcgaacgatc cgaacgtgga tcccataggt
      841 gcctgcatcg gtgaaggagg atcgaggata gccgccatac tgaaggagct caaggg
//
