LOCUS       sequence-96449-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows the integration of Myc ArB K106R in the genome and 
            Tet-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Girdler F, Gascoigne KE, Eyers PA, Hartmuth S, Crafter C, Foote KM, 
            Keen NJ, Taylor SS
  TITLE     Validating Aurora B as an anti-cancer drug target.
  JOURNAL   J Cell Sci. 2006 Sep 1;119(Pt 17):3664-75. Epub 2006 Aug 15.
  PUBMED    16912073
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2006-09-1"; volume: "119(Pt 17)"; pages: "3664-75. 
            Epub 2006 Aug 15"
COMMENT     Sequence Label: pcDNA5 TO FRT Myc ArB K106R
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             331..360
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccccgg ggaattcggc
      181 agatcccgag gtccgacagc gcccggccca gatccccacg cctgccagga gcaagccgag
      241 agccagccgg ccggcgcact ccgactccga gcagtctctg tccttcgacc cgagccccgc
      301 gccctttccg ggacccctgc cccgcggatg gagcagaagc tcatctcaga agaagacctc
      361 gaggcccaga aggagaactc ctacccctgg ccctacggcc gacagacggc tccatctggc
      421 ctgagcaccc tgccccagcg agtcctccgg aaagagcctg tcaccccatc tgcacttgtc
      481 ctcatgagcc gctccaatgt ccagcccaca gctgcccctg gccagaaggt gatggagaat
      541 agcagtggga cacccgacat cttaacgcgg cacttcacaa ttgatgactt tgagattggg
      601 cgtcctctgg gcaaaggcaa gtttggaaac gtgtacttgg ctcgggagaa gaaaagccat
      661 ttcatcgtgg cgctcagggt cctcttcaag tcccagatag agaaggaggg cgtggagcat
      721 cagctgcgca gagagatcga aatccaggcc cacctgcacc atcccaacat cctgcgtctc
      781 tacaactatt tttatgaccg gaggaggatc tacttgattc tagagtatgc cccccgcggg
      841 gagctctaca aggagctgca gaagagctgc aca
//
