LOCUS       sequence-96453-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK194
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgatcgcaag gcccgtacgc acaatcctta caagctttca tcaaccttga ccaacattta
       61 tacaggttcc agactccacg aagccggatg tgcaccctca tatcatgtgg tgcgagggga
      121 tattgccacg gccaccgaag gagtgattat aaatgctgct aacagcaaag gacaacctgg
      181 cggaggggtg tgcggagcgc tgtataagaa attcccggaa agcttcgatt tacagccgat
      241 cgaagtagga aaagcgcgac tggtcaaagg tgcagctaaa catatcattc atgccgtagg
      301 accaaacttc aacaaagttt cggaggttga aggtgacaaa cagttggcag aggcttatga
      361 gtccatcgct aagattgtca acgataacaa ttacaagtca gtagcgattc cactgttgtc
      421 caccggcatc ttttccggga acaaagatcg actaacccaa tcattgaacc atttgctgac
      481 agctttagac accactgatg cagatgtagc catatactgc agggacaaga aatgggaaat
      541 gactctcaag gaagcagtgg ctaggagaga agcagtggag gagatatgca tatccgacga
      601 ctcttcagtg acagaacctg atgcagagct ggtgagggtg catccgaaga gttctttggc
      661 tggaaggaag ggctacagca caagcgatgg caaaactttc tcatatttgg aagggaccaa
      721 gtttcaccag gcggccaagg atatagcaga aattaatgcc atgtggcccg ttgcaacgga
      781 ggccaatgag caggtatgca tgtatatcct cggagaaagc atgagcagta ttaggtcgaa
      841 atgccccgtc gaagagtcgg aagcctccac accacctagc acgctgcctt gcttgtgcat
      901 ccatgccatg actccagaa
//
