LOCUS       sequence-96454-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak 
            mKate opal attB2 AscI (truncated E1) 3'UTR Poly A I-SceI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Wroblewska L, Kitada T, Endo K, Siciliano V, Stillo B, Saito H, 
            Weiss R
  TITLE     Mammalian synthetic circuits with RNA binding proteins for RNA-only 
            delivery.
  JOURNAL   Nat Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301.
  PUBMED    26237515
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Aug 3. doi: 10.1038/nbt.3301."
COMMENT     Sequence Label: pTK194
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(23..47)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(580..599)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggggcac aaacttaatt gaacccagct ttcttgtaca aagtggtggc gcgcctatgt
       61 tacgtgcaaa ggtgattgtc accccccgaa agaccatatt gtgacacacc ctcagtatca
      121 cgcccaaaca tttacagccg cggtgtcaaa aaccgcgtgg acgtggttaa catccctgct
      181 gggaggatca gccgtaatta ttataattgg cttggtgctg gctactattg tggccatgta
      241 cgtgctgacc aaccagaaac ataattgaat acagcagcaa ttggcaagct gcttacatag
      301 aactcgcggc gattggcatg ccgccttaaa atttttattt tatttttctt ttcttttccg
      361 aatcggattt tgtttttaat atttcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      421 aaaaataggg ataacagggt aattgagcaa aaggccagca aaaggccagg aaccgtaaaa
      481 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      541 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      601 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      661 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      721 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accc
//
