LOCUS       sequence-96465-e         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV 
            promoter). Also suitable for in vitro transcription (T7 promoter) 
            for injecting mRNA into oocytes. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-ELYS-emerin-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gttaagcttc gaattcatgc aagacttgac agctcaagtg actagtgatc
      121 tcctgcattt cccagaagtg actattgaag ctcttggaga agatgagata acattagagt
      181 ccgtgcttcg tggaaagttt gctgcaggga aaaatggact agcatgctta gcttgtggtc
      241 cacaacttga agttgtaaac tccttaacag gagagcggtt atctgcatat agattcagtg
      301 gagtaaatga acagcctcct gtagtccttg cagtgaaaga attctcttgg cataagagga
      361 ctggattgtt aataggattg gaagaagcag atgggagtgt tctttgtctt tatgaccttg
      421 gtatatcaag agtggtcaaa gcagttgttc ttcctggaag ggtaacagct atcgagccta
      481 taattaacca tggaggagcc agtgcgagta cccagcattt acatccaagt ctccggtggc
      541 tttttggcgt ggccgctgtg gtgactgatg ttggacagat ccttcttatt gacctg
//
