LOCUS       sequence-96466-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable 
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes. This LBR contains N-terminal domain only..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-LBR-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt cgaattcatg ccaagtagga agtttgttga gggtgaagtg
      121 gtcagaggcc gatggcctgg gagctccctt tattatgaag tagagattct gagccacgac
      181 aacaaatccc agctctacac tgtaaaatac aaagatggca ccgaactgga actgaaggag
      241 agtgatatca agcctttaaa atcctttaag caaagaaaaa gtggctcgat ttccagctct
      301 ccctctcgac gccgtggcag ccgcagccgc agccgctcaa gatcacgttc ccgatccccg
      361 ggcagagcac cgaaaggctc cagaagatcc gtctctgctt cccatgaggg tgacgtgaag
      421 gagaagaagg agaaggagat gaggagggaa attctgcaag ttaagctgac tccgcttgtg
      481 ttgaagccgt tcggaaacag cgtcagcgtg tacaacgggg agcccgaaca catggagaag
      541 aacgctacgc cgtataaaga caagcaggaa agaatcattt tgtcaacaga agacagatat
      601 atagttacac agtacagcct tcgtcccagg agagaagagg tcaaagccaa agaaatagag
      661 tctgaggaac aaaaccttgt caccaaagga cccgcacctt tgggaacctt tcaagtgacc
      721 actccacaga ggaaggactt ggagtttgga ggagtacctg gtgcggtcct catcatgctg
      781 ggcctgcccg cctgcgtcct cttgctgctg ctgcagtgca ggcagaa
//
