LOCUS       sequence-96468-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Flag-tagged RCC1 in mammalian cells (CMV promoter). Also 
            suitable for in vitro transcription (T7 promoter) for injecting mRNA
            into oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-Flag-RCC1-polyA
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt atggactaca aagacgatga cgacaaggga tccccaccca
      121 agcgcatagc taagagaagg tcacccccag aagatgccat ccccaaaagc aagaaggtga
      181 aagtctcaca caggtcccac aacacagaac caggcttggt gctgacactg ggccagggcg
      241 acgtgggcca gctggggctg ggtgaaagtg tgctggagag gaagaagccg gccttggtgc
      301 cccttctgca ggatgttgtg caggctgagg ccgggggcat gcatactgtg tgtctgagcc
      361 aaagtggcca ggtctactcc tttggctgca atgacgaggg tgccctggga agggacactt
      421 cagtcgaagg ctcagaaatg gtccctggga aagtggaact gcaagagaaa gtggtgcaag
      481 tgtcagcggg ggacagtcac acagcagccc tcaccgaaga cggccgtgtc ttcctttggg
      541 gctctttccg ggacaataat ggtgtgatcg ggttgttgga gcccatgaag aagagcatgg
      601 tacctgttca agtgcagctg gatgcgcccg tggtaaaggt ggcctcgggg aacgaccact
      661 tggtgatgct cacgaacgat ggcgacctct acaccttggg ttgtggggag caaggtcagc
      721 tgggccgtgt acctgaatta tttgccaacc gaggtggccg tcagggcctt gggcgactcc
      781 tggtccccag atgtgtgctg ctgaaatcca gg
//
