LOCUS       sequence-96470-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV 
            promoter). Also suitable for in vitro transcription (T7 promoter) 
            for injecting mRNA into oocytes. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-ELYS-emerin-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgctcac catggtggcg accggtggat ccctgggatc catccagaag gggttgcctt
       61 cttcagcttg tatggaatag taaacaaaga gcagaaaggc agcaaagacc aggaagagta
      121 acagctggcc ccagagtggg acctgtcgat cctggcccag agcagcagca ggggcctgct
      181 tctctggccg aatggcacgt cgggtaagcc atgaagaggg agaagatgaa gaggaggaca
      241 cagaagaggt ggaggatgta ggataatatg ataagcccag agagctcctg gagacattgg
      301 aaatggggcg gtagtgtgcg atgctctggt aggcactgtc ttggccatag atgaggcgct
      361 ccctatcctt gccttcctct tctaaagaag agaaaatgtc atcacgcacc tggtgatgga
      421 aggtatcagc atctacaagt gaggtccccg gctggcggaa gctcttggac atgcccacag
      481 actcaggctc cccgtatgtc ttggtggtca aataactctc ctcatagtag tcatcattat
      541 agtccttgct ctggtaaagt aaggcgtctt cttttttggg cagatcatac atgtctgagt
      601 ccacggcggc tgaatccaag tctgagaact gataggagaa tgaagaagat gacgagttgg
      661 ggggtaagag tctccttctc tgg
//
