LOCUS       sequence-96477-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express wild-type form of Ran-mRFP1 in mammalian cells (CMV 
            promoter). Also suitable for in vitro transcription (T7 promoter) 
            for injecting mRNA into oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-RanWT-mRFP1-polyA
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt atggccgccc agggagagcc gcaggtccag ttcaagctcg
      121 tcctggtggg cgacggcggc accgggaaga caaccttcgt gaagcgccac ttgacgggcg
      181 agtttgagaa gaagtatgta gccaccctgg gcgtggaggt gcacccgctc gtcttccata
      241 ccaacagagg acccatcaag ttcaacgtgt gggacacggc cggccaggag aagttcgggg
      301 gcctgcgcga tggctactac atccaagccc agtgtgccat tataatgttt gatgtaacct
      361 caagagttac ttacaagaat gtacctaact ggcatagaga tctggtacga gtgtgtgaaa
      421 acatccccat tgtattgtgt ggcaacaaag tggatattaa agacaggaaa gtgaaggcaa
      481 aatctattgt cttccaccgg aagaagaatc ttcagtacta tgacatttct gccaaaagta
      541 actacaactt tgaaaagcct ttcctctggc ttgccagaaa gctcattgga gatcctaact
      601 tggagtttgt tgccatgcct gctcttgccc cacctgaggt ggtcatggac ccagctttgg
      661 cagcacagta cgagcatgat ttagaggttg ctcagacgac tgctctccca gatgaggatg
      721 atgacctgga tccaatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg
      781 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcg
//
