LOCUS       sequence-96481-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). 
            Also suitable for in vitro transcription (T7 promoter) for injecting
            mRNA into oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-Flag-H3.3-polyA
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..107
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt atggactaca aagacgatga cgacaaggga tccactagta
      121 acggccgcca gtgtgctgga attcacgccc gaaccaagca gactgctcgt aagtccaccg
      181 gtgggaaagc cccccgcaaa cagctggcca cgaaagccgc caggaaaagc gctccctcta
      241 ccggcggggt gaagaagcct catcgctaca ggcccgggac cgtggcgctt cgagagattc
      301 gtcgttatca gaagtcgacc gagctgctca tccggaagct gcccttccag aggttggtga
      361 gggagatcgc gcaggatttc aaaaccgacc tgaggtttca gagcgcagcc atcggtgcgc
      421 tgcaggaggc tagcgaagcg tacctggtgg gtctgttcga agataccaac ctgtgtgcca
      481 tccacgctaa gagagtcacc atcatgccca aagacatcca gttggctcgc cggatacggg
      541 gagagagagc ttaagaattc tgcagatatc catcacactg gcggccgctc gactatgggc
      601 accaaagaac ctgtaaacgt tatcttttta aattgaatgt gcacaaataa aagtttggaa
      661 a
//
