LOCUS       sequence-96483-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also 
            suitable for in vitro transcription (T7 promoter) for injecting mRNA
            into oocytes. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-HIRA-polyA
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt cgaattcatg aagctcttga agccaacctg ggtcaaccac
      121 aatgggaagc caattttttc agttgatatt caccctgatg ggaccaagtt tgcaactgga
      181 ggacaagggc aggattctgg gaaggttgtg atctggaata tgtctccagt cctccaggag
      241 gatgacgaga aggatgaaaa tattcccaag atgctttgcc agatggacaa tcacttagca
      301 tgtgtgaact gtgtgcggtg gtcaaacagt gggatgtatt tagcttctgg gggagatgac
      361 aaactgatta tggtgtggaa gcgggctacg tacattgggc ccagcactgt gtttggttcc
      421 agtggtaagc ttgccaatgt ggagcaatgg cggtgtgtct ccatcctccg gagtcactca
      481 ggcgatgtga tggatgtagc atggtctccc cacgacgcct ggctggcctc atgcagcgtg
      541 gataacactg ttgtcatttg gaatgccgtg aagttcccag aaattcttgc aactctgaga
      601 ggtcattctg gcttagtaaa aggtttgact tgggatcccg ttggtaaata tattgcctct
      661 caagctgatg atcgaagttt gaaggtatgg aggacgctgg actggcagct agagactagc
      721 atcaccaagc cttttgatga gtgtggagga acgacgcatg ttctccggct tagttggtca
      781 cctgatggcc attacctggt atctgcccat gccatgaata attctggccc
//
