LOCUS       sequence-96491-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable
            for in vitro transcription (T7 promoter) for injecting mRNA into 
            oocytes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Inoue A, Zhang Y
  TITLE     Nucleosome assembly is required for nuclear pore complex assembly in
            mouse zygotes.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):609-16. doi: 10.1038/nsmb.2839. 
            Epub 2014 Jun 8.
  PUBMED    24908396
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2839";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "609-16"
COMMENT     Sequence Label: pcDNA-emerin-EGFP-polyA
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt cgaattcatg gacgactatg cggttttgtc cgatactgag
      121 ctggccgcag tgctacgcca gtacaacatc cctcatgggc ctattgtggg ttccactcgc
      181 aagctctacg aaaagaaaat cttcgagtac gagacccaga gaaggagact cttacccccc
      241 aactcgtcat cttcttcatt ctcctatcag ttctcagact tggattcagc cgccgtggac
      301 tcagacatgt atgatctgcc caaaaaagaa gacgccttac tttaccagag caaggactat
      361 aatgatgact actatgagga gagttatttg accaccaaga catacgggga gcctgagtct
      421 gtgggcatgt ccaagagctt ccgccagccg gggacctcac ttgtagatgc tgataccttc
      481 catcaccagg tgcgtgatga cattttctct tctttagaag aggaaggcaa ggatagggag
      541 cgcctcatct atggccaaga cagtgcctac cagagcatcg cacactaccg ccccatttcc
      601 aatgtctcca ggagctctct gggcttatca tattatccta catcctccac ctcttctgtg
      661 tcctcctctt catcttctcc ctcttcatgg cttacccgac gtgccattcg gccagagaag
      721 caggcccctg ctgctgctct gggccaggat cgacaggtcc cactctgggg ccagctgtta
      781 ctcttcctgg tctttgctgc ctttctgctc tttgtttact attccataca agctgaagaa
      841 ggcaacc
//
