LOCUS       sequence-96498-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding PKA KTR mClover flanked by gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     284..305
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(566..665)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccctcgaact tcacctcggc gcgggtcttg taggtaccgt cgtccttgaa agagatggtg
       61 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      121 tcggggtagc ggctgaagca ggccacgccg tagccgaagg tggtcacgag ggtgggccag
      181 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gttggtggca
      241 tcgccctcgc cctcgccgcg gacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      301 accaggatgg gcacaacccc ggtgaacagc tcctcgccct tgctcaccat actagtgctg
      361 gactggaggg tcaggcgctc cagccttgac gacacctgac ccgacgcctt ccttctagat
      421 ggcgcctcgt cttcgttgac catactggcc cgtttgggga ccttcacaag ggagtcgcag
      481 atgctgacat actcgggact gtaaatataa accgggggta gtgaatgccc actgttggcg
      541 ggttcctctg gcatctccat ggtggagcct gcttttttgt acaaagttgg cattataaaa
      601 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
      661 atttggggcc cgagcttaag taactaacta acaggaagag tttgta
//
