LOCUS       sequence-96499-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding PKA KTR mClover flanked by gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-PKAKTRClover
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(367..388)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctccac catggagatg
      121 ccagaggaac ccgccaacag tgggcattca ctacccccgg tttatattta cagtcccgag
      181 tatgtcagca tctgcgactc ccttgtgaag gtccccaaac gggccagtat ggtcaacgaa
      241 gacgaggcgc catctagaag gaaggcgtcg ggtcaggtgt cgtcaaggct ggagcgcctg
      301 accctccagt ccagcactag tatggtgagc aagggcgagg agctgttcac cggggttgtg
      361 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt ccgcggcgag
      421 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
      481 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc ctgcttcagc
      541 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      601 gtccaggagc gcaccatctc tttcaaggac gacggtacct acaagacccg cgccgaggtg
      661 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      721 gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc
      781 acggccgaca agcag
//
