LOCUS       sequence-96500-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-p38KTRmCerulean3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     284..305
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(545..644)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(663..686)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(694..721)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
       61 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
      121 tcggggtagc gggcgaagca ctgcacgccc cagctcaggg tggtcacgag ggtgggccag
      181 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
      241 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
      301 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat actagtgctg
      361 gactggaggg tcaggcgctc cagccttgac gacacctccg gcgacgccag cttcttagat
      421 ggcgactcgt cttcgttgag accggccgag cgcttggagt tatttatcct ttgattcact
      481 gatggcatcg tgttcttgct gccaggtggg ataagaacgc ggagatctgg cttacgcatg
      541 gtggagcctg cttttttgta caaagttggc attataaaaa agcattgctt atcaatttgt
      601 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttggggccc gagcttaagt
      661 aactaactaa caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      721 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      781 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      841 gacaa
//
