LOCUS       sequence-96501-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Regot S, Hughey JJ, Bajar BT, Carrasco S, Covert MW
  TITLE     High-sensitivity measurements of multiple kinase activities in live 
            single cells.
  JOURNAL   Cell. 2014 Jun 19;157(7):1724-34. doi: 10.1016/j.cell.2014.04.039.
  PUBMED    24949979
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.04"; journalName: "Cell"; date: "2014-06-19- 
            19"; volume: "157"; issue: "7"; pages: "1724-34"
COMMENT     Sequence Label: pENTR-p38KTRmCerulean3
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    7..106
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(346..367)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(607..626)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
       61 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctccac catgcgtaag
      121 ccagatctcc gcgttcttat cccacctggc agcaagaaca cgatgccatc agtgaatcaa
      181 aggataaata actccaagcg ctcggccggt ctcaacgaag acgagtcgcc atctaagaag
      241 ctggcgtcgc cggaggtgtc gtcaaggctg gagcgcctga ccctccagtc cagcactagt
      301 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      361 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      421 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      481 ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      541 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      601 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      661 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      721 aagctggagt acaacgccat ccacggcaac gtctatatca ccgccgacaa gcagaagaac
      781 ggcatcaagg ccaacttcgg cctgaactgc aacatcgagg acggcagcgt gcagctcgcc
      841 gaccactacc agcagaacac ccc
//
