LOCUS       sequence-96506-e        1593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria-targeted genetically encoded fluorescent indicator for 
            measuring NAD/NADH ratio..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, 
            Belousov VV
  TITLE     Genetically encoded fluorescent indicator for imaging NAD/NADH ratio
            changes in different cellular compartments.
  JOURNAL   Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018.
  PUBMED    24286672
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 
            10.1016/j.bbagen.2013.11.018."
COMMENT     Sequence Label: pC1-mitoRexYFP
FEATURES             Location/Qualifiers
     source          1..1593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..168
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     669..690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(799..820)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1060..1079)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgggg gatctgtccg tcctgacgcc gctgctgctg
      121 cggggcttga caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg
      181 ggggatccac cggtcgccac cgagatgaag gtccccgaag cggccatcag ccgcctgatc
      241 acctacctcc gcatcctgga ggagctggag gcccaggggg ttcaccggac gagctccgag
      301 cagcttgggg agctcgccca ggtcacggcc ttccaggtgc ggaaggacct ttcctacttc
      361 ggctcctacg ggacgcgcgg ggtggggtac accgtccccg tcctgaagcg ggagctccgc
      421 cacatcctgg ggctcaaccg ctccgccggc tacaacagcg acaacgtcta tatcatggcc
      481 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacgt cgaggacggc
      541 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      601 ctgcccgaca accactacct gagcttccag tccgtcctga gcaaagaccc caacgagaag
      661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactcc cggcatggac
      721 gagctgaaca acgtggatgg cggtagcggt ggcaccggca gcaagggcga ggagctgttc
      781 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      841 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
      901 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctcgg ctacggcctg
      961 aagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1021 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1081 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1141 ggcttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgg caccaagtgg
     1201 ggcctgtgca tcgtgggcat gggccggctc gggagcgccc tggccgacta cccggggttc
     1261 ggggagagct ttgagctcag gggcttcttt gacgtggacc cggagaaggt gggccgcccg
     1321 gtgcggggcg gggtcattga gcacgtggac ctcctgcccc agcgggttcc cggccgcatt
     1381 gagatcgccc tcctcaccgt gccccgggaa gcggcccaga aggcggcgga cctgttggtg
     1441 gcggcgggga tcaaggggat cctgaacttc gccccggtgg tcctcgaggt gcccaaggag
     1501 gtggcggtgg agaacgtgga cttcttggcg gggctcacgc ggctttcttt cgctatcctg
     1561 aaccccaagt ggcgggaaga gatgatgggg tga
//
