LOCUS       sequence-96508-e        1626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mitochondria-targeted genetically encoded hydrogen peroxide 
            indicator with red fluorescence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, 
            Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G, Belousov VV
  TITLE     Red fluorescent genetically encoded indicator for intracellular 
            hydrogen peroxide.
  JOURNAL   Nat Commun. 2014 Oct 21;5:5222. doi: 10.1038/ncomms6222.
  PUBMED    25330925
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-21"; volume: "5:5222. doi"; pages: " 
            10.1038/ncomms6222"
COMMENT     Sequence Label: pC1-HyPerRed-mito
FEATURES             Location/Qualifiers
     source          1..1626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..168
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             join(202..579,580..588,589..1314,1315..1323,1324..1626)
                     /codon_start=1
                     /product="red fluorescent genetically encoded indicator for
                     H2O2 ()"
                     /label=HyPerRed
                     /translation="EMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYL
                     HEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPM
                     ADLAGEKLLMLEDGHCLRDQAMPRTVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVKT
                     TYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGSL
                     VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLP
                     FAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQ
                     DGVFIYKVKLRGTNFPPDGPVMQKKTMGWEAAGVGFCFEAGADEDTHFRATSLETLRNM
                     VAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEAI
                     RARMDGHFDKVLKQAV"
     primer_bind     1278..1297
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgtccgtcc tgacgccgct gctgctgcgg ggcttgacag gctcggcccg gcggctccca
       61 gtgccgcgcg ccaagatcca ttcgttgggg gatctgtccg tcctgacgcc gctgctgctg
      121 cggggcttga caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg
      181 ggggatccac cggtcgccac cgagatggcg agccagcagg gcgagacgat gtccggaccg
      241 ctgcacattg gtttgattcc cacagttgga ccgtacctgc taccgcatat tatccctatg
      301 ctgcaccaga cctttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
      361 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcactggt gaaagagagc
      421 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagat
      481 cacccgtggg cgaaccgcga atgcgtaccg atggccgatc tggcagggga aaaactgctg
      541 atgctggaag atggtcactg tttgcgcgat caggcaatgc cccgcacggt ttccgagcgg
      601 atgtaccccg aggacggcgc cctgaagagc gagatcaaga aggggctgag gctgaaggac
      661 ggcggccact acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      721 cccggcgcct acatcgtcga catcaagttg gacatcgtgt cccacaacga ggactacacc
      781 atcgtggaac agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      841 tacaagggag gtacaggcgg gagtctggtg agcaagggcg aggaggataa catggccatc
      901 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      961 gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc taagctgaag
     1021 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     1081 ggctccaagg cctacattaa gcacccagcc gacatccccg actacttcaa gctgtccttc
     1141 cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat tattcacgtt
     1201 aaccaggact cctccctgca ggacggcgta ttcatctaca aggtgaagct gcgcggcacc
     1261 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggctgccggg
     1321 gtgggtttct gttttgaagc cggggcggat gaagatacac acttccgcgc gaccagcctg
     1381 gaaactctgc gcaacatggt ggcggcaggt agcgggatca ctttactgcc agcgctggct
     1441 gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc cgtgcattaa gccggaacca
     1501 cgccgcacta ttggtctggt ttatcgtcct ggctcaccgc tgcgcagccg ctatgagcag
     1561 ctggcagagg ccatccgcgc aagaatggat ggccatttcg ataaagtttt aaaacaggcg
     1621 gtttaa
//
