LOCUS       sequence-96510-e        1668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 
            targeted to mitochondrial intermembrane spase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, 
            Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G, Belousov VV
  TITLE     Red fluorescent genetically encoded indicator for intracellular 
            hydrogen peroxide.
  JOURNAL   Nat Commun. 2014 Oct 21;5:5222. doi: 10.1038/ncomms6222.
  PUBMED    25330925
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-21"; volume: "5:5222. doi"; pages: " 
            10.1038/ncomms6222"
COMMENT     Sequence Label: IMS-HyPer2
FEATURES             Location/Qualifiers
     source          1..1668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             235..1668
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="EMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYL
                     HEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPM
                     ADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQL
                     ADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELY
                     NVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTT
                     GKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLETL
                     RNMVAVGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLA
                     EAIRARMDGHFDKVLKQAV"
     primer_bind     840..861
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(970..991)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1231..1250)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggcggctc tgaaaagttg gctgtcgcgc agcgtaactt cattcttcag gtacagacag
       61 tgtttgtgtg ttcctgttgt ggctaacttt aaaaagcggt gtttctcaaa attgataaga
      121 ccatggcaca aaactgtgac gattggcttt ggagtaaccc tgtgtgcggt tcctattaag
      181 cttcaaattc tgcagtcaac ggtaccgcgg gcccgggatc cacccgtcgc caccgagatg
      241 gcaagccagc agggcgagac gatgtccgga ccgctgcaca ttggtttgat tcccacagtt
      301 ggaccgtacc tgctaccgca tattatccct atgctgcacc agacctttcc aaagctggaa
      361 atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc
      421 gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt
      481 gatgagccaa tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta
      541 ccgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc
      601 gatcaggcaa tgtccgccgg ctacaacagc gacaacgtct atatcatggc cgacaagcag
      661 aagaacggca tcaaggccaa cttcaagatc cgccacaacg tcgaggacgg cagcgtgcag
      721 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      781 aaccactacc tgagcttcca gtccgtcctg agcaaagacc ccaacgagaa gcgcgatcac
      841 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      901 aacgtggatg gcggtagcgg tggcaccggc agcaagggcg aggagctgtt caccggggtg
      961 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1021 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     1081 aagctgcccg tgccctggcc caccctcgtg accaccctcg gctacggcct gaagtgcttc
     1141 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1201 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1261 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cggcttcaag
     1321 gaggacggca acatcctggg gcacaagctg gagtacaacg gcaccggttt ctgttttgaa
     1381 gccggggcgg atgaagatac acacttccgc gcgaccagcc tggaaactct gcgcaacatg
     1441 gtggcggtag gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa
     1501 cgcgatgggg ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggcctg
     1561 gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccgc
     1621 gcaagaatgg atggccattt cgataaagtt ttaaaacagg cggtttaa
//
