LOCUS       sequence-96512-e        3249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains a human pancreatic islet inactive enhancer, 
            which can be shuttled into a Gateway destination vector, such as 
            pGL4.23-Gateway..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Pasquali L, Gaulton KJ, Rodriguez-Segui SA, Mularoni L, 
            Miguel-Escalada I, Akerman I, Tena JJ, Moran I, Gomez-Marin C, van 
            de Bunt M, Ponsa-Cobas J, Castro N, Nammo T, Cebola I, 
            Garcia-Hurtado J, Maestro MA, Pattou F, Piemonti L, Berney T, Gloyn 
            AL, Ravassard P, Gomez-Skarmeta JL, Muller F, McCarthy MI, Ferrer J
  TITLE     Pancreatic islet enhancer clusters enriched in type 2 diabetes 
            risk-associated variants.
  JOURNAL   Nat Genet. 2014 Feb;46(2):136-43. doi: 10.1038/ng.2870. Epub 2014 
            Jan 12.
  PUBMED    24413736
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.2870"; 
            journalName: "Nat Genet"; date: "2014-02"; volume: "46"; issue: "2";
            pages: "136-43"
COMMENT     Sequence Label: pENTR D-TOPO-C2_8
FEATURES             Location/Qualifiers
     source          1..3249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1374..1473)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1490..1509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1491..1509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1514..1530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1514..1530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1632..1651)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1643..2452
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1710..1729)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2599..3187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3088..3107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccgg aaatgtatca agattccaga attgataaac
      721 ttagctatac ttggcataat ttagtacacc tgaccaaatg tatcttttaa ggaaaataat
      781 gttttggtca acctagaaag tattagtcat ggccttaaat tttttctgca tggtttacaa
      841 attttttgtt tgtctgcaat gataatagtt atactgaaga atattcactt tacttaagta
      901 gatggtaata catttaaaaa gcagtctttc atttccattt cacttagtca aacatttact
      961 ggtgatgtgc ttttgatgat ttgctatgtt gctaaaacag gatatgcatt cactgttttt
     1021 tgacagctca tggatccaag agcacatgta gttcctaaag gcacagagta taggatattt
     1081 ttggatactg tgatattccc ccctttcttc tggtaattag aagcattccc agtattaaac
     1141 atttaggagg aaaagttata gtcaaagaag accatctctt tcttttaaat cattttaata
     1201 tgaagcatat attttacttt agagctttaa aatgatttaa agtaacaatt ttagtaacct
     1261 ttgggtttgt cttgaaagta ttttcatttc atttgtttct aaaatacttg tttctctgaa
     1321 gagagaagat ggctcaatct gatgacatac gtaaccgaag ggtgggcgcg ccgacccagc
     1381 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1441 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     1501 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1561 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1621 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1681 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1741 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1801 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1861 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1921 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     1981 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2041 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2101 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2161 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2221 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2281 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2341 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2401 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2461 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     2521 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2701 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     3001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3241 tcacatgtt
//
